1nh6: Difference between revisions

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OCA

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-[[Image:1nh6.gif|left|200px]]<br /><applet load="1nh6" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1nh6, resolution 2.05&Aring;" />
-'''Structure of S. marcescens chitinase A, E315L, complex with hexasaccharide'''<br />
-==Overview==
+==Structure of S. marcescens chitinase A, E315L, complex with hexasaccharide==
-The sizes and anomers of the products formed during the hydrolysis of, chitin oligosaccharides by the Family 18 chitinase A (ChiA) from Serratia, marcescens were analysed by hydrophilic interaction chromatography using a, novel approach in which reactions were performed at 0 degrees C to, stabilize the anomer conformations of the initial products., Crystallographic studies of the enzyme, having the structure of the, complex of the ChiA E315L (Glu315--&gt;Leu) mutant with a hexasaccharide, show that the oligosaccharide occupies subsites -4 to +2 in the, substrate-binding cleft, consistent with the processing of beta-chitin by, the release of disaccharide at the reducing end. Products of the, hydrolysis of hexa- and penta-saccharides by wild-type ChiA, as well as by, two mutants of the residues Trp275 and Phe396 important in binding the, substrate at the +1 and +2 sites, show that the substrates only occupy, sites -2 to +2 and that additional N -acetyl-D-glucosamines extend beyond, the substrate-binding cleft at the reducing end. The subsites -3 and -4, are not used in this four-site binding mode. The explanation for these, results is found in the high importance of individual binding sites for, the processing of short oligosaccharides compared with the cumulative, recognition and processive hydrolysis mechanism used to digest natural, beta-chitin.
+<StructureSection load='1nh6' size='340' side='right'caption='[[1nh6]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1nh6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NH6 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nh6 OCA], [https://pdbe.org/1nh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nh6 RCSB], [https://www.ebi.ac.uk/pdbsum/1nh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nh6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CHIA_SERMA CHIA_SERMA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nh/1nh6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nh6 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The sizes and anomers of the products formed during the hydrolysis of chitin oligosaccharides by the Family 18 chitinase A (ChiA) from Serratia marcescens were analysed by hydrophilic interaction chromatography using a novel approach in which reactions were performed at 0 degrees C to stabilize the anomer conformations of the initial products. Crystallographic studies of the enzyme, having the structure of the complex of the ChiA E315L (Glu315--&gt;Leu) mutant with a hexasaccharide, show that the oligosaccharide occupies subsites -4 to +2 in the substrate-binding cleft, consistent with the processing of beta-chitin by the release of disaccharide at the reducing end. Products of the hydrolysis of hexa- and penta-saccharides by wild-type ChiA, as well as by two mutants of the residues Trp275 and Phe396 important in binding the substrate at the +1 and +2 sites, show that the substrates only occupy sites -2 to +2 and that additional N -acetyl-D-glucosamines extend beyond the substrate-binding cleft at the reducing end. The subsites -3 and -4 are not used in this four-site binding mode. The explanation for these results is found in the high importance of individual binding sites for the processing of short oligosaccharides compared with the cumulative recognition and processive hydrolysis mechanism used to digest natural beta-chitin.
-==About this Structure==
+Family 18 chitinase-oligosaccharide substrate interaction: subsite preference and anomer selectivity of Serratia marcescens chitinase A.,Aronson NN Jr, Halloran BA, Alexyev MF, Amable L, Madura JD, Pasupulati L, Worth C, Van Roey P Biochem J. 2003 Nov 15;376(Pt 1):87-95. PMID:12932195<ref>PMID:12932195</ref>
-NH6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Active as [http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NH6 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Family 18 chitinase-oligosaccharide substrate interaction: subsite preference and anomer selectivity of Serratia marcescens chitinase A., Aronson NN Jr, Halloran BA, Alexyev MF, Amable L, Madura JD, Pasupulati L, Worth C, Van Roey P, Biochem J. 2003 Nov 15;376(Pt 1):87-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12932195 12932195]
+</div>
-[[Category: Chitinase]]
+<div class="pdbe-citations 1nh6" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Chitinase 3D structures|Chitinase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Serratia marcescens]]
-[[Category: Single protein]]
+[[Category: Alexyev MF]]
-[[Category: Alexyev, M.F.]]
+[[Category: Amable L]]
-[[Category: Amable, L.]]
+[[Category: Aronson Jr NN]]
-[[Category: Halloran, B.A.]]
+[[Category: Halloran BA]]
-[[Category: Jr., N.N.Aronson.]]
+[[Category: Madura JD]]
-[[Category: Madura, J.D.]]
+[[Category: Pasupulati L]]
-[[Category: Pasupulati, L.]]
+[[Category: Van Roey P]]
-[[Category: Roey, P.Van.]]
+[[Category: Worth C]]
-[[Category: Worth, C.]]
-[[Category: (beta/alpha)8-barrel]]
-[[Category: oligosaccharide complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:15:04 2007''