1ndp: Difference between revisions

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OCA

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-[[Image:1ndp.jpg|left|200px]]<br /><applet load="1ndp" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1ndp, resolution 2.2&Aring;" />
-'''ADENOSINE 5'-DIPHOSPHATE BINDING AND THE ACTIVE SITE OF NUCLEOSIDE DIPHOSPHATE KINASE'''<br />
-==Overview==
+==ADENOSINE 5'-DIPHOSPHATE BINDING AND THE ACTIVE SITE OF NUCLEOSIDE DIPHOSPHATE KINASE==
-The X-ray structure of nucleoside diphosphate kinase (NDP kinase) from the, slime mold Dictyostelium discoideum has been determined to 2.2-A, resolution and refined to an R-factor of 0.19 with and without bound, ADP-Mg2+. The nucleotide binds near His 122, a residue which becomes, phosphorylated during the catalytic cycle. The mode of binding is, different from that observed in other phosphokinases, and it involves no, glycine-rich sequence. The adenine base makes only nonpolar contacts with, the protein. It points outside, explaining the lack of specificity of NDP, kinase toward the base. The ribose 2'- and 3'-hydroxyls and the, pyrophosphate moiety are H-bonded to polar side chains. A Mg2+ ion bridges, the alpha- to the beta-phosphate which approaches the imidazole group of, His 122 from the N delta side. The geometry at the active site in the, ADP-Mg2+ complex suggests a mechanism for catalysis whereby the, gamma-phosphate of a nucleoside triphosphate can be transferred onto His, 122 with a minimum of atomic motion.
+<StructureSection load='1ndp' size='340' side='right'caption='[[1ndp]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ndp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NDP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NDP FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ndp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ndp OCA], [https://pdbe.org/1ndp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ndp RCSB], [https://www.ebi.ac.uk/pdbsum/1ndp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ndp ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NDKC_DICDI NDKC_DICDI]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nd/1ndp_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ndp ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-NDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NDP OCA].
+*[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Adenosine 5'-diphosphate binding and the active site of nucleoside diphosphate kinase., Morera S, Lascu I, Dumas C, LeBras G, Briozzo P, Veron M, Janin J, Biochemistry. 1994 Jan 18;33(2):459-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8286376 8286376]
 [[Category: Dictyostelium discoideum]]
-[[Category: Nucleoside-diphosphate kinase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Dumas C]]
-[[Category: Dumas, C.]]
+[[Category: Janin J]]
-[[Category: Janin, J.]]
+[[Category: Lascu I]]
-[[Category: Lascu, I.]]
+[[Category: Lebras G]]
-[[Category: Lebras, G.]]
+[[Category: Morera S]]
-[[Category: Morera, S.]]
+[[Category: Veron M]]
-[[Category: Veron, M.]]
-[[Category: ADP]]
-[[Category: MG]]
-[[Category: phosphotransferase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:10:09 2007''