1n4j: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /><applet load="1n4j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n4j, resolution 2.18Å" /> '''STREPTAVIDIN MUTANT ...
 
No edit summary
 
(16 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1n4j.gif|left|200px]]<br /><applet load="1n4j" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1n4j, resolution 2.18&Aring;" />
'''STREPTAVIDIN MUTANT N23A AT 2.18A'''<br />


==Overview==
==STREPTAVIDIN MUTANT N23A AT 2.18A==
An elaborate hydrogen-bonding network contributes to the tight binding of, biotin to streptavidin. The specific energetic contributions of hydrogen, bonds to the biotin ureido oxygen have previously been investigated by, mapping the equilibrium and activation thermodynamic signatures of N23A, N23E, S27A, Y43A and Y43F site-directed mutants [Klumb et al. (1998), Biochemistry, 37, 7657-7663]. The crystal structures of these variants in, the unbound and biotin-bound states provide structural insight into the, energetic alterations and are described here. High (1.5-2.2 A) to atomic, resolution (1.14 A) structures were obtained and structural models were, refined to R values ranging from 0.12 to 0.20. The overall folding of, streptavidin as described previously has not changed in any of the mutant, structures. Major deviations such as side-chain shifts of residues in the, binding site are observed only for the N23A and Y43A mutations. In none of, the mutants is a systematic shift of biotin observed when one of the, hydrogen-bonding partners to the ureido oxygen of biotin is removed., Recent thermodynamic studies report increases of DeltaDeltaG(o) of, 5.0-14.6 kJ mol(-1) for these mutants with respect to the wild-type, protein. The decreasing stabilities of the complexes of the mutants are, discussed in terms of their structures.
<StructureSection load='1n4j' size='340' side='right'caption='[[1n4j]], [[Resolution|resolution]] 2.18&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1n4j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N4J FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.18&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4j OCA], [https://pdbe.org/1n4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n4j RCSB], [https://www.ebi.ac.uk/pdbsum/1n4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4j ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n4/1n4j_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n4j ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1N4J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N4J OCA].
*[[Avidin 3D structures|Avidin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin., Le Trong I, Freitag S, Klumb LA, Chu V, Stayton PS, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1567-73. Epub 2003, Aug 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12925786 12925786]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Streptomyces avidinii]]
[[Category: Streptomyces avidinii]]
[[Category: Chu, V.]]
[[Category: Chu V]]
[[Category: Freitag, S.]]
[[Category: Freitag S]]
[[Category: Klumb, L.A.]]
[[Category: Klumb LA]]
[[Category: Stayton, P.S.]]
[[Category: Le Trong I]]
[[Category: Stenkamp, R.E.]]
[[Category: Stayton PS]]
[[Category: Trong, I.Le.]]
[[Category: Stenkamp RE]]
[[Category: homotetramer]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:57:20 2007''

Latest revision as of 10:52, 14 February 2024

STREPTAVIDIN MUTANT N23A AT 2.18ASTREPTAVIDIN MUTANT N23A AT 2.18A

Structural highlights

1n4j is a 1 chain structure with sequence from Streptomyces avidinii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.18Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SAV_STRAV The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1n4j, resolution 2.18Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1n4j&oldid=4052173"