2og0: Difference between revisions

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-{{Seed}}
-[[Image:2og0.png|left|200px]]
-<!--
+==Crystal Structure of the Lambda Xis-DNA complex==
-The line below this paragraph, containing "STRUCTURE_2og0", creates the "Structure Box" on the page.
+<StructureSection load='2og0' size='340' side='right'caption='[[2og0]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2og0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OG0 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [https://pdbe.org/2og0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [https://www.ebi.ac.uk/pdbsum/2og0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2og0 ProSAT]</span></td></tr>
-{{STRUCTURE_2og0|  PDB=2og0  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The phage-encoded Xis protein is the major determinant controlling the direction of recombination in phage lambda. Xis is a winged-helix DNA binding protein that cooperatively binds to the attR recombination site to generate a curved microfilament, which promotes assembly of the excisive intasome but inhibits formation of an integrative intasome. We find that lambda synthesizes surprisingly high levels of Xis immediately upon prophage induction when excision rates are maximal. However, because of its low sequence-specific binding activity, exemplified by a 1.9 A co-crystal structure of a non-specifically bound DNA complex, Xis is relatively ineffective at promoting excision in vivo in the absence of the host Fis protein. Fis binds to a segment in attR that almost entirely overlaps one of the Xis binding sites. Instead of sterically excluding Xis binding from this site, as has been previously believed, we show that Fis enhances binding of all three Xis protomers to generate the microfilament. A specific Fis-Xis interface is supported by the effects of mutations within each protein, and relaxed, but not completely sequence-neutral, binding by the central Xis protomer is supported by the effects of DNA mutations. We present a structural model for the 50 bp curved Fis-Xis cooperative complex that is assembled between the arm and core Int binding sites whose trajectory places constraints on models for the excisive intasome structure.
-===Crystal Structure of the Lambda Xis-DNA complex===
+Fis targets assembly of the Xis nucleoprotein filament to promote excisive recombination by phage lambda.,Papagiannis CV, Sam MD, Abbani MA, Yoo D, Cascio D, Clubb RT, Johnson RC J Mol Biol. 2007 Mar 23;367(2):328-43. Epub 2007 Jan 3. PMID:17275024<ref>PMID:17275024</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_17275024}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2og0" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 17275024 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_17275024}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Escherichia virus Lambda]]
-OG0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA].
+[[Category: Large Structures]]
+[[Category: Abbani MA]]
-==Reference==
+[[Category: Cascio D]]
-Fis targets assembly of the Xis nucleoprotein filament to promote excisive recombination by phage lambda., Papagiannis CV, Sam MD, Abbani MA, Yoo D, Cascio D, Clubb RT, Johnson RC, J Mol Biol. 2007 Mar 23;367(2):328-43. Epub 2007 Jan 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17275024 17275024]
+[[Category: Clubb RT]]
-[[Category: Enterobacteria phage lambda]]
+[[Category: Johnson RC]]
-[[Category: Single protein]]
+[[Category: Papagiannis CV]]
-[[Category: Abbani, M A.]]
+[[Category: Sam MD]]
-[[Category: Cascio, D.]]
+[[Category: Yoo D]]
-[[Category: Clubb, R T.]]
-[[Category: Johnson, R C.]]
-[[Category: Papagiannis, C V.]]
-[[Category: Sam, M D.]]
-[[Category: Yoo, D.]]
-[[Category: Winged helix protein]]
-[[Category: Dna architectural protein]]
-[[Category: Phage excision]]
-[[Category: Protein-dna complex]]
-[[Category: Site-specific recombination recombination]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:56:07 2008''