2fl5: Difference between revisions

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-{{Seed}}
-[[Image:2fl5.png|left|200px]]
-<!--
+==Cofactor-containing antibodies: Crystal structure of the original yellow antibody==
-The line below this paragraph, containing "STRUCTURE_2fl5", creates the "Structure Box" on the page.
+<StructureSection load='2fl5' size='340' side='right'caption='[[2fl5]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2fl5]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FL5 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
-{{STRUCTURE_2fl5|  PDB=2fl5  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fl5 OCA], [https://pdbe.org/2fl5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fl5 RCSB], [https://www.ebi.ac.uk/pdbsum/2fl5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fl5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q5FWF9_HUMAN Q5FWF9_HUMAN]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2fl5_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fl5 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Antibodies are generally thought to be a class of proteins that function without the use of cofactors. However, it is not widely appreciated that antibodies are believed to be the major carrier protein in human circulation for the important riboflavin cofactor that is involved in a host of biological phenomena. A further link between riboflavin and antibodies was discovered 30 years ago when a bright-yellow antibody, IgG(GAR), was purified from a patient with multiple myeloma who had turned yellow during the course of her disease. It was subsequently shown that the yellow color of this antibody was due to riboflavin binding. However, it was not known how and where riboflavin was bound to this antibody. We now report the crystal structure of this historically important IgG(GAR) Fab at 3.0-A resolution. The riboflavin is located in the antigen-combining site with its isoalloxazine ring stacked between the parallel aromatic moieties of TyrH33, PheH58, and TyrH100A. Together with additional hydrogen bonds, these interactions reveal the structural basis for high-affinity riboflavin binding. The ligand specificity of IgG(GAR) is compared with another riboflavin-binding antibody, IgG(DOT), which was purified from a second patient with multiple myeloma. The crystal structure of IgG(GAR) provides a starting point for attempts to understand the physiological relevance and chemical functions of cofactor-containing antibodies.
-===Cofactor-containing antibodies: Crystal structure of the original yellow antibody===
+Cofactor-containing antibodies: crystal structure of the original yellow antibody.,Zhu X, Wentworth P Jr, Kyle RA, Lerner RA, Wilson IA Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3581-5. Epub 2006 Feb 28. PMID:16537445<ref>PMID:16537445</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_16537445}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2fl5" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 16537445 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16537445}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Homo sapiens]]
-Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL5 OCA].
+[[Category: Large Structures]]
+[[Category: Wilson IA]]
-==Reference==
+[[Category: Zhu X]]
-Cofactor-containing antibodies: crystal structure of the original yellow antibody., Zhu X, Wentworth P Jr, Kyle RA, Lerner RA, Wilson IA, Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3581-5. Epub 2006 Feb 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16537445 16537445]
-[[Category: Wilson, I A.]]
-[[Category: Zhu, X.]]
-[[Category: Cofactor]]
-[[Category: Human antibody]]
-[[Category: Igggar]]
-[[Category: Multiple myeloma]]
-[[Category: Riboflavin-binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:41:09 2008''