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New page: left|200px<br /><applet load="1mew" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mew, resolution 2.15Å" /> '''Inosine Monophosphat...
 
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[[Image:1mew.jpg|left|200px]]<br /><applet load="1mew" size="450" color="white" frame="true" align="right" spinBox="true"
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'''Inosine Monophosphate Dehydrogenase (IMPDH) From Tritrichomonas Foetus with XMP and NAD bound'''<br />


==Overview==
==Inosine Monophosphate Dehydrogenase (IMPDH) From Tritrichomonas Foetus with XMP and NAD bound==
The enzyme inosine monophosphate dehydrogenase (IMPDH) is responsible for, the rate-limiting step in guanine nucleotide biosynthesis. Because it is, up-regulated in rapidly proliferating cells, human type II IMPDH is, actively targeted for immunosuppressive, anticancer, and antiviral, chemotherapy. The enzyme employs a random-in ordered-out kinetic mechanism, where substrate or cofactor can bind first but product is only released, after the cofactor leaves. Due to structural and kinetic differences, between mammalian and microbial enzymes, most drugs that are successful in, the inhibition of mammalian IMPDH are far less effective against the, microbial forms of the enzyme. It is possible that with greater knowledge, of the structural mechanism of the microbial enzymes, an effective and, selective inhibitor of microbial IMPDH will be developed for use as a drug, against multi-drug resistant bacteria and protists. The high-resolution, crystal structures of four different complexes of IMPDH from the protozoan, parasite Tritrichomonas foetus have been solved: with its substrate IMP, IMP and the inhibitor mycophenolic acid (MPA), the product XMP with MPA, and XMP with the cofactor NAD(+). In addition, a potassium ion has been, located at the dimer interface. A structural model for the kinetic, mechanism is proposed.
<StructureSection load='1mew' size='340' side='right'caption='[[1mew]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mew]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tritrichomonas_suis Tritrichomonas suis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MEW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=XMP:XANTHOSINE-5-MONOPHOSPHATE'>XMP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mew OCA], [https://pdbe.org/1mew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mew RCSB], [https://www.ebi.ac.uk/pdbsum/1mew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mew ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IMDH_TRIFO IMDH_TRIFO] Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism.<ref>PMID:10029522</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/me/1mew_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mew ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MEW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Tritrichomonas_foetus Tritrichomonas foetus] with K, XMP and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/IMP_dehydrogenase IMP dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.205 1.1.1.205] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MEW OCA].
*[[Inosine monophosphate dehydrogenase 3D structures|Inosine monophosphate dehydrogenase 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structures of Tritrichomonasfoetus inosine monophosphate dehydrogenase in complex with substrate, cofactor and analogs: a structural basis for the random-in ordered-out kinetic mechanism., Prosise GL, Luecke H, J Mol Biol. 2003 Feb 14;326(2):517-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12559919 12559919]
__TOC__
[[Category: IMP dehydrogenase]]
</StructureSection>
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Tritrichomonas foetus]]
[[Category: Tritrichomonas suis]]
[[Category: Luecke, H.]]
[[Category: Luecke H]]
[[Category: Prosise, G.L.]]
[[Category: Prosise GL]]
[[Category: K]]
[[Category: NAD]]
[[Category: XMP]]
[[Category: alpha beta barrel]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:22:35 2007''

Latest revision as of 11:35, 10 April 2024

Inosine Monophosphate Dehydrogenase (IMPDH) From Tritrichomonas Foetus with XMP and NAD boundInosine Monophosphate Dehydrogenase (IMPDH) From Tritrichomonas Foetus with XMP and NAD bound

Structural highlights

1mew is a 1 chain structure with sequence from Tritrichomonas suis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.15Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IMDH_TRIFO Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Digits JA, Hedstrom L. Kinetic mechanism of Tritrichomonas foetus inosine 5'-monophosphate dehydrogenase. Biochemistry. 1999 Feb 23;38(8):2295-306. PMID:10029522 doi:http://dx.doi.org/10.1021/bi982305k

1mew, resolution 2.15Å

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