1m4z: Difference between revisions

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New page: left|200px<br /><applet load="1m4z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m4z, resolution 2.2Å" /> '''Crystal structure of ...
 
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[[Image:1m4z.jpg|left|200px]]<br /><applet load="1m4z" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1m4z, resolution 2.2&Aring;" />
'''Crystal structure of the N-terminal BAH domain of Orc1p'''<br />


==Overview==
==Crystal structure of the N-terminal BAH domain of Orc1p==
The N-terminal domain of the largest subunit of the Saccharomyces, cerevisiae origin recognition complex (Orc1p) functions in transcriptional, silencing and contains a bromo-adjacent homology (BAH) domain found in, some chromatin-associated proteins including Sir3p. The 2.2 A crystal, structure of the N-terminal domain of Orc1p revealed a BAH core and a, non-conserved helical sub-domain. Mutational analyses demonstrated that, the helical sub-domain was necessary and sufficient to bind Sir1p, and, critical for targeting Sir1p primarily to the cis-acting E silencers at, the HMR and HML silent chromatin domains. In the absence of the BAH, domain, approximately 14-20% of cells in a population were silenced at the, HML locus. Moreover, the distributions of the Sir2p, Sir3p and Sir4p, proteins, while normal, were at levels lower than found in wild-type, cells. Thus, in the absence of the Orc1p BAH domain, HML resembled, silencing of genes adjacent to telomeres. These data are consistent with, the view that the Orc1p-Sir1p interaction at the E silencers ensures, stable inheritance of pre-established Sir2p, Sir3p and Sir4p complexes at, the silent mating type loci.
<StructureSection load='1m4z' size='340' side='right'caption='[[1m4z]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1m4z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M4Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M4Z FirstGlance]. <br>
1M4Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M4Z OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m4z OCA], [https://pdbe.org/1m4z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m4z RCSB], [https://www.ebi.ac.uk/pdbsum/1m4z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m4z ProSAT]</span></td></tr>
Structure and function of the BAH-containing domain of Orc1p in epigenetic silencing., Zhang Z, Hayashi MK, Merkel O, Stillman B, Xu RM, EMBO J. 2002 Sep 2;21(17):4600-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12198162 12198162]
</table>
== Function ==
[https://www.uniprot.org/uniprot/ORC1_YEAST ORC1_YEAST] Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.<ref>PMID:17825064</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m4/1m4z_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m4z ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Hayashi MK]]
[[Category: Hayashi, M.K.]]
[[Category: Merkel O]]
[[Category: Merkel, O.]]
[[Category: Stillman B]]
[[Category: Stillman, B.]]
[[Category: Xu R-M]]
[[Category: Xu, R.M.]]
[[Category: Zhang Z]]
[[Category: Zhang, Z.]]
[[Category: MN]]
[[Category: bah domain]]
[[Category: chromatin]]
[[Category: dna replication]]
[[Category: transcriptional silencing]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:09:06 2007''

Latest revision as of 10:39, 14 February 2024

Crystal structure of the N-terminal BAH domain of Orc1pCrystal structure of the N-terminal BAH domain of Orc1p

Structural highlights

1m4z is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ORC1_YEAST Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Asano T, Makise M, Takehara M, Mizushima T. Interaction between ORC and Cdt1p of Saccharomyces cerevisiae. FEMS Yeast Res. 2007 Dec;7(8):1256-62. Epub 2007 Sep 6. PMID:17825064 doi:http://dx.doi.org/10.1111/j.1567-1364.2007.00299.x

1m4z, resolution 2.20Å

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