1lz8: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1lz8.jpg|left|200px]]<br /><applet load="1lz8" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1lz8, resolution 1.53&Aring;" />
-'''LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES'''<br />
-==Overview==
+==LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES==
-A general method for solving the phase problem from native crystals of, macromolecules has long eluded structural biology. For well diffracting, crystals this goal can now be achieved, as is shown here, thanks to modern, data collection techniques and new statistical phasing algorithms. Using, solely a native crystal of tetragonal hen egg-white lysozyme, a protein of, 14 kDa molecular mass, it was possible to detect the positions of the ten, sulfur and seven chlorine atoms from their anomalous signal, and proceed, from there to obtain an electron-density map of very high quality.
+<StructureSection load='1lz8' size='340' side='right'caption='[[1lz8]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1lz8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LZ8 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.53&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lz8 OCA], [https://pdbe.org/1lz8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lz8 RCSB], [https://www.ebi.ac.uk/pdbsum/1lz8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lz8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lz/1lz8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lz8 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A general method for solving the phase problem from native crystals of macromolecules has long eluded structural biology. For well diffracting crystals this goal can now be achieved, as is shown here, thanks to modern data collection techniques and new statistical phasing algorithms. Using solely a native crystal of tetragonal hen egg-white lysozyme, a protein of 14 kDa molecular mass, it was possible to detect the positions of the ten sulfur and seven chlorine atoms from their anomalous signal, and proceed from there to obtain an electron-density map of very high quality.
-==About this Structure==
+Can anomalous signal of sulfur become a tool for solving protein crystal structures?,Dauter Z, Dauter M, de La Fortelle E, Bricogne G, Sheldrick GM J Mol Biol. 1999 May 28;289(1):83-92. PMID:10339407<ref>PMID:10339407</ref>
-LZ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with CL and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LZ8 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Can anomalous signal of sulfur become a tool for solving protein crystal structures?, Dauter Z, Dauter M, de La Fortelle E, Bricogne G, Sheldrick GM, J Mol Biol. 1999 May 28;289(1):83-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10339407 10339407]
+</div>
+<div class="pdbe-citations 1lz8" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Lysozyme 3D structures|Lysozyme 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Gallus gallus]]
-[[Category: Lysozyme]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Bricogne G]]
-[[Category: Bricogne, G.]]
+[[Category: Dauter M]]
-[[Category: Dauter, M.]]
+[[Category: Dauter Z]]
-[[Category: Dauter, Z.]]
+[[Category: De La Fortelle E]]
-[[Category: Fortelle, E.De.La.]]
+[[Category: Sheldrick GM]]
-[[Category: Sheldrick, G.M.]]
-[[Category: CL]]
-[[Category: NA]]
-[[Category: glycosidase]]
-[[Category: hydrolase]]
-[[Category: o-glycosyl]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:00:13 2007''