2gmo: Difference between revisions
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< | ==NMR-structure of an independently folded C-terminal domain of influenza polymerase subunit PB2== | ||
<StructureSection load='2gmo' size='340' side='right'caption='[[2gmo]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2gmo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Victoria/3/1975(H3N2)) Influenza A virus (A/Victoria/3/1975(H3N2))]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GMO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gmo OCA], [https://pdbe.org/2gmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gmo RCSB], [https://www.ebi.ac.uk/pdbsum/2gmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gmo ProSAT]</span></td></tr> | ||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PB2_I75A3 PB2_I75A3] Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The trimeric influenza virus polymerase, comprising subunits PA, PB1 and PB2, is responsible for transcription and replication of the segmented viral RNA genome. Using a novel library-based screening technique called expression of soluble proteins by random incremental truncation (ESPRIT), we identified an independently folded C-terminal domain from PB2 and determined its solution structure by NMR. Using green fluorescent protein fusions, we show that both the domain and the full-length PB2 subunit are efficiently imported into the nucleus dependent on a previously overlooked bipartite nuclear localization sequence (NLS). The crystal structure of the domain complexed with human importin alpha5 shows how the last 20 residues unfold to permit binding to the import factor. The domain contains three surface residues implicated in adaptation from avian to mammalian hosts. One of these tethers the NLS-containing peptide to the core of the domain in the unbound state. | |||
Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit.,Tarendeau F, Boudet J, Guilligay D, Mas PJ, Bougault CM, Boulo S, Baudin F, Ruigrok RW, Daigle N, Ellenberg J, Cusack S, Simorre JP, Hart DJ Nat Struct Mol Biol. 2007 Mar;14(3):229-33. Epub 2007 Feb 25. PMID:17310249<ref>PMID:17310249</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2gmo" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | [[Category: Large Structures]] | ||
[[Category: Boudet J]] | |||
[[Category: Bougault CM]] | |||
== | [[Category: Cusack S]] | ||
[[Category: Guilligay D]] | |||
[[Category: Hart DJ]] | |||
[[Category: | [[Category: Mas P]] | ||
[[Category: Boudet | [[Category: Simorre J-P]] | ||
[[Category: Bougault | [[Category: Tarendeau F]] | ||
[[Category: Cusack | |||
[[Category: Guilligay | |||
[[Category: Hart | |||
[[Category: Mas | |||
[[Category: Simorre | |||
[[Category: Tarendeau | |||
Latest revision as of 19:36, 13 December 2023
NMR-structure of an independently folded C-terminal domain of influenza polymerase subunit PB2NMR-structure of an independently folded C-terminal domain of influenza polymerase subunit PB2
Structural highlights
FunctionPB2_I75A3 Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication (By similarity). Publication Abstract from PubMedThe trimeric influenza virus polymerase, comprising subunits PA, PB1 and PB2, is responsible for transcription and replication of the segmented viral RNA genome. Using a novel library-based screening technique called expression of soluble proteins by random incremental truncation (ESPRIT), we identified an independently folded C-terminal domain from PB2 and determined its solution structure by NMR. Using green fluorescent protein fusions, we show that both the domain and the full-length PB2 subunit are efficiently imported into the nucleus dependent on a previously overlooked bipartite nuclear localization sequence (NLS). The crystal structure of the domain complexed with human importin alpha5 shows how the last 20 residues unfold to permit binding to the import factor. The domain contains three surface residues implicated in adaptation from avian to mammalian hosts. One of these tethers the NLS-containing peptide to the core of the domain in the unbound state. Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit.,Tarendeau F, Boudet J, Guilligay D, Mas PJ, Bougault CM, Boulo S, Baudin F, Ruigrok RW, Daigle N, Ellenberg J, Cusack S, Simorre JP, Hart DJ Nat Struct Mol Biol. 2007 Mar;14(3):229-33. Epub 2007 Feb 25. PMID:17310249[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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