1kx1: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1kx1.gif|left|200px]]<br /><applet load="1kx1" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1kx1, resolution 2.8&Aring;" />
-'''Rat mannose protein A complexed with Man6-GlcNAc2-Asn'''<br />
-==Overview==
+==Rat mannose protein A complexed with Man6-GlcNAc2-Asn==
-Mannose-binding proteins (MBPs) are C-type animal lectins that recognize, high mannose oligosaccharides on pathogenic cell surfaces. MBPs bind to, their carbohydrate ligands by forming a series of Ca(2+) coordination and, hydrogen bonds with two hydroxyl groups equivalent to the 3- and 4-OH of, mannose. In this work, the determinants of the orientation of sugars bound, to rat serum and liver MBPs (MBP-A and MBP-C) have been systematically, investigated. The crystal structures of MBP-A soaked with monosaccharides, and disaccharides and also the structure of the MBP-A trimer cross-linked, by a high mannose asparaginyl oligosaccharide reveal that monosaccharides, or alpha1-6-linked mannose bind to MBP-A in one orientation, whereas, alpha1-2- or alpha1-3-linked mannose binds in an orientation rotated 180, degrees around a local symmetry axis relating the 3- and 4-OH groups. In, contrast, a similar set of ligands all bind to MBP-C in a single, orientation. The mutation of MBP-A His(189) to its MBP-C equivalent, valine, causes Man alpha 1-3Man to bind in a mixture of orientations., These data combined with modeling indicate that the residue at this, position influences the orientation of bound ligands in MBP. We propose, that the control of binding orientation can influence the recognition of, multivalent ligands. A lateral association of trimers in the cross-linked, crystals may reflect interactions within higher oligomers of MBP-A that, are stabilized by multivalent ligands.
+<StructureSection load='1kx1' size='340' side='right'caption='[[1kx1]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1kx1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KX1 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kx1 OCA], [https://pdbe.org/1kx1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kx1 RCSB], [https://www.ebi.ac.uk/pdbsum/1kx1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kx1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MBL1_RAT MBL1_RAT] Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kx/1kx1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kx1 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-KX1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MAN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KX1 OCA].
+*[[Mannose-binding protein|Mannose-binding protein]]
+__TOC__
-==Reference==
+</StructureSection>
-Orientation of bound ligands in mannose-binding proteins. Implications for multivalent ligand recognition., Ng KK, Kolatkar AR, Park-Snyder S, Feinberg H, Clark DA, Drickamer K, Weis WI, J Biol Chem. 2002 May 3;277(18):16088-95. Epub 2002 Feb 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11850428 11850428]
+[[Category: Large Structures]]
 [[Category: Rattus norvegicus]]
-[[Category: Single protein]]
+[[Category: Clark DA]]
-[[Category: Clark, D.A.]]
+[[Category: Drickamer K]]
-[[Category: Drickamer, K.]]
+[[Category: Feinberg H]]
-[[Category: Feinberg, H.]]
+[[Category: Kolatkar AR]]
-[[Category: Kolatkar, A.R.]]
+[[Category: Ng KK]]
-[[Category: Ng, K.K.]]
+[[Category: Park-Snyder S]]
-[[Category: Park-Snyder, S.]]
+[[Category: Weis WI]]
-[[Category: Weis, W.I.]]
-[[Category: CA]]
-[[Category: MAN]]
-[[Category: c-type lectin]]
-[[Category: calcium-binding protein]]
-[[Category: lectin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:02:13 2007''