2v6u: Difference between revisions

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-{{Seed}}
-[[Image:2v6u.png|left|200px]]
-<!--
+==High resolution crystal structure of pterin-4a-carbinolamine dehydratase from Toxoplasma gondii==
-The line below this paragraph, containing "STRUCTURE_2v6u", creates the "Structure Box" on the page.
+<StructureSection load='2v6u' size='340' side='right'caption='[[2v6u]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2v6u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii_RH Toxoplasma gondii RH]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V6U FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-{{STRUCTURE_2v6u|  PDB=2v6u  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6u OCA], [https://pdbe.org/2v6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v6u RCSB], [https://www.ebi.ac.uk/pdbsum/2v6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v6u ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q2Q449_TOXGO Q2Q449_TOXGO]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v6/2v6u_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v6u ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produced a high-resolution structure (1.6A) of the apo-enzyme and a low-resolution structure (3.1A) of a complex with a substrate-like ligand dihydrobiopterin (BH(2)). Analysis of the hydrogen bonding interactions that contribute to binding BH(2) suggest that the ligand is present in an enol tautomeric state, which makes it more similar to the physiological substrate. The enzyme can process (R)- and (S)-forms of pterin-4a-carbinolamine and the ligand complex suggests that His61 and His79 are placed to act independently as general bases for catalysis of the individual enantiomers. Comparisons with orthologues from other protozoan parasites (Plasmodium falciparum and Leishmania major) and with rat PCD, for which the structure is known, indicate a high degree of sequence and structure conservation of this enzyme. The molecular determinants of ligand recognition and PCD reactivity are therefore highly conserved across species.
-===HIGH RESOLUTION CRYSTAL STRUCTURE OF PTERIN-4A-CARBINOLAMINE DEHYDRATASE FROM TOXOPLASMA GONDII===
+Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues.,Cameron S, Fyffe SA, Goldie S, Hunter WN Mol Biochem Parasitol. 2008 Apr;158(2):131-8. Epub 2007 Dec 15. PMID:18215430<ref>PMID:18215430</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_18215430}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2v6u" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 18215430 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18215430}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-V6U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6U OCA].
+[[Category: Toxoplasma gondii RH]]
+[[Category: Cameron S]]
-==Reference==
+[[Category: Goldie SP]]
-Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues., Cameron S, Fyffe SA, Goldie S, Hunter WN, Mol Biochem Parasitol. 2008 Apr;158(2):131-8. Epub 2007 Dec 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18215430 18215430]
+[[Category: Hunter WN]]
-[[Category: 4a-hydroxytetrahydrobiopterin dehydratase]]
-[[Category: Single protein]]
-[[Category: Toxoplasma gondii]]
-[[Category: Cameron, S.]]
-[[Category: Goldie, S P.]]
-[[Category: Hunter, W N.]]
-[[Category: Dehydratase]]
-[[Category: Enzyme]]
-[[Category: Lyase]]
-[[Category: Pterin]]
-[[Category: Toxoplasma]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:46:19 2008''