4lzm: Difference between revisions

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New page: left|200px<br /><applet load="4lzm" size="450" color="white" frame="true" align="right" spinBox="true" caption="4lzm, resolution 1.7Å" /> '''COMPARISON OF THE CRY...
 
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[[Image:4lzm.jpg|left|200px]]<br /><applet load="4lzm" size="450" color="white" frame="true" align="right" spinBox="true"
caption="4lzm, resolution 1.7&Aring;" />
'''COMPARISON OF THE CRYSTAL STRUCTURE OF BACTERIOPHAGE T4 LYSOZYME AT LOW, MEDIUM, AND HIGH IONIC STRENGTHS'''<br />


==Overview==
==COMPARISON OF THE CRYSTAL STRUCTURE OF BACTERIOPHAGE T4 LYSOZYME AT LOW, MEDIUM, AND HIGH IONIC STRENGTHS==
Crystals of bacteriophage T4 lysozyme used for structural studies are, routinely grown from concentrated phosphate solutions. It has been found, that crystals in the same space group can also be grown from solutions, containing 0.05 M imidazole chloride, 0.4 M sodium choride, and 30%, polyethylene glycol 3500. These crystals, in addition, can also be, equilibrated with a similar mother liquor in which the sodium chloride, concentration is reduced to 0.025 M. The availability of these three, crystal variants has permitted the structure of T4 lysozyme to be compared, at low, medium, and high ionic strength. At the same time the X-ray, structure of phage T4 lysozyme crystallized from phosphate solutions has, been further refined against a new and improved X-ray diffraction data, set. The structures of T4 lysozyme in the crystals grown with polyethylene, glycol as a precipitant, regardless of the sodium chloride concentration, were very similar to the structure in crystals grown from concentrated, phosphate solutions. The main differences are related to the formation of, mixed disulfides between cysteine residues 54 and 97 and, 2-mercaptoethanol, rather than to the differences in the salt, concentration in the crystal mother liquor. Formation of the mixed, disulfide at residue 54 resulted in the displacement of Arg-52 and the, disruption of the salt bridge between this residue and Glu-62. Other than, this change, no obvious alterations in existing salt bridges in T4, lysozyme were observed. Neither did the reduction in the ionic strength of, the mother liquor result in the formation of new salt bridge interactions., These results are consistent with the ideas that a crystal structure, determined at high salt concentrations is a good representation of the, structure at lower ionic strengths, and that models of electrostatic, interactions in proteins that are based on crystal structures determined, at high salt concentrations are likely to be relevant at physiological, ionic strengths.
<StructureSection load='4lzm' size='340' side='right'caption='[[4lzm]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4lzm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LZM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lzm OCA], [https://pdbe.org/4lzm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lzm RCSB], [https://www.ebi.ac.uk/pdbsum/4lzm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lzm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lz/4lzm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4lzm ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
4LZM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with CL and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4LZM OCA].
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
 
== References ==
==Reference==
<references/>
Comparison of the crystal structure of bacteriophage T4 lysozyme at low, medium, and high ionic strengths., Bell JA, Wilson KP, Zhang XJ, Faber HR, Nicholson H, Matthews BW, Proteins. 1991;10(1):10-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2062826 2062826]
__TOC__
[[Category: Bacteriophage t4]]
</StructureSection>
[[Category: Lysozyme]]
[[Category: Escherichia virus T4]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bell, J.A.]]
[[Category: Bell JA]]
[[Category: Faber, H.R.]]
[[Category: Faber HR]]
[[Category: Matthews, B.W.]]
[[Category: Matthews BW]]
[[Category: Nicholson, H.]]
[[Category: Nicholson H]]
[[Category: Wilson, K.]]
[[Category: Wilson K]]
[[Category: Zhang, X.J.]]
[[Category: Zhang X-J]]
[[Category: BME]]
[[Category: CL]]
[[Category: hydrolase (o-glycosyl)]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:41:31 2007''

Latest revision as of 15:22, 1 March 2024

COMPARISON OF THE CRYSTAL STRUCTURE OF BACTERIOPHAGE T4 LYSOZYME AT LOW, MEDIUM, AND HIGH IONIC STRENGTHSCOMPARISON OF THE CRYSTAL STRUCTURE OF BACTERIOPHAGE T4 LYSOZYME AT LOW, MEDIUM, AND HIGH IONIC STRENGTHS

Structural highlights

4lzm is a 1 chain structure with sequence from Escherichia virus T4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENLYS_BPT4 Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Moussa SH, Kuznetsov V, Tran TA, Sacchettini JC, Young R. Protein determinants of phage T4 lysis inhibition. Protein Sci. 2012 Apr;21(4):571-82. doi: 10.1002/pro.2042. Epub 2012 Mar 2. PMID:22389108 doi:http://dx.doi.org/10.1002/pro.2042

4lzm, resolution 1.70Å

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