1kpl: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1kpl.gif|left|200px]]<br /><applet load="1kpl" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1kpl, resolution 3.00&Aring;" />
-'''Crystal Structure of the ClC Chloride Channel from S. typhimurium'''<br />
-==Overview==
+==Crystal Structure of the ClC Chloride Channel from S. typhimurium==
-The ClC chloride channels catalyse the selective flow of Cl- ions across, cell membranes, thereby regulating electrical excitation in skeletal, muscle and the flow of salt and water across epithelial barriers. Genetic, defects in ClC Cl- channels underlie several familial muscle and kidney, diseases. Here we present the X-ray structures of two prokaryotic ClC Cl-, channels from Salmonella enterica serovar typhimurium and Escherichia coli, at 3.0 and 3.5 A, respectively. Both structures reveal two identical, pores, each pore being formed by a separate subunit contained within a, homodimeric membrane protein. Individual subunits are composed of two, roughly repeated halves that span the membrane with opposite orientations., This antiparallel architecture defines a selectivity filter in which a Cl-, ion is stabilized by electrostatic interactions with alpha-helix dipoles, and by chemical coordination with nitrogen atoms and hydroxyl groups., These findings provide a structural basis for further understanding the, function of ClC Cl- channels, and establish the physical and chemical, basis of their anion selectivity.
+<StructureSection load='1kpl' size='340' side='right'caption='[[1kpl]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1kpl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KPL FirstGlance]. <br>
-KPL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with CL, SO4, MYS and OCT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KPL OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MYS:PENTADECANE'>MYS</scene>, <scene name='pdbligand=OCT:N-OCTANE'>OCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kpl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kpl OCA], [https://pdbe.org/1kpl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kpl RCSB], [https://www.ebi.ac.uk/pdbsum/1kpl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kpl ProSAT]</span></td></tr>
-X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity., Dutzler R, Campbell EB, Cadene M, Chait BT, MacKinnon R, Nature. 2002 Jan 17;415(6869):287-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11796999 11796999]
+</table>
-[[Category: Salmonella typhimurium]]
+== Function ==
-[[Category: Single protein]]
+[https://www.uniprot.org/uniprot/CLCA_SALTY CLCA_SALTY] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:11796999</ref>
-[[Category: Cadene, M.]]
+== Evolutionary Conservation ==
-[[Category: Campbell, E.B.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Chait, B.T.]]
+Check<jmol>
-[[Category: Dutzler, R.]]
+  <jmolCheckbox>
-[[Category: MacKinnon, R.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kp/1kpl_consurf.spt"</scriptWhenChecked>
-[[Category: CL]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: MYS]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: OCT]]
+  </jmolCheckbox>
-[[Category: SO4]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kpl ConSurf].
-[[Category: helical membrane protein]]
+<div style="clear:both"></div>
-[[Category: homodimer]]
+== References ==
-[[Category: ion channel]]
+<references/>
+__TOC__
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:35:13 2007''
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
+[[Category: Cadene M]]
+[[Category: Campbell EB]]
+[[Category: Chait BT]]
+[[Category: Dutzler R]]
+[[Category: MacKinnon R]]