1nh6: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:1nh6.png|left|200px]]
-<!--
+==Structure of S. marcescens chitinase A, E315L, complex with hexasaccharide==
-The line below this paragraph, containing "STRUCTURE_1nh6", creates the "Structure Box" on the page.
+<StructureSection load='1nh6' size='340' side='right'caption='[[1nh6]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1nh6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NH6 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-{{STRUCTURE_1nh6|  PDB=1nh6  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nh6 OCA], [https://pdbe.org/1nh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nh6 RCSB], [https://www.ebi.ac.uk/pdbsum/1nh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nh6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CHIA_SERMA CHIA_SERMA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nh/1nh6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nh6 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The sizes and anomers of the products formed during the hydrolysis of chitin oligosaccharides by the Family 18 chitinase A (ChiA) from Serratia marcescens were analysed by hydrophilic interaction chromatography using a novel approach in which reactions were performed at 0 degrees C to stabilize the anomer conformations of the initial products. Crystallographic studies of the enzyme, having the structure of the complex of the ChiA E315L (Glu315--&gt;Leu) mutant with a hexasaccharide, show that the oligosaccharide occupies subsites -4 to +2 in the substrate-binding cleft, consistent with the processing of beta-chitin by the release of disaccharide at the reducing end. Products of the hydrolysis of hexa- and penta-saccharides by wild-type ChiA, as well as by two mutants of the residues Trp275 and Phe396 important in binding the substrate at the +1 and +2 sites, show that the substrates only occupy sites -2 to +2 and that additional N -acetyl-D-glucosamines extend beyond the substrate-binding cleft at the reducing end. The subsites -3 and -4 are not used in this four-site binding mode. The explanation for these results is found in the high importance of individual binding sites for the processing of short oligosaccharides compared with the cumulative recognition and processive hydrolysis mechanism used to digest natural beta-chitin.
-===Structure of S. marcescens chitinase A, E315L, complex with hexasaccharide===
+Family 18 chitinase-oligosaccharide substrate interaction: subsite preference and anomer selectivity of Serratia marcescens chitinase A.,Aronson NN Jr, Halloran BA, Alexyev MF, Amable L, Madura JD, Pasupulati L, Worth C, Van Roey P Biochem J. 2003 Nov 15;376(Pt 1):87-95. PMID:12932195<ref>PMID:12932195</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1nh6" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_12932195}}, adds the Publication Abstract to the page
+*[[Chitinase 3D structures|Chitinase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 12932195 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_12932195}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-NH6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NH6 OCA].
-==Reference==
-Family 18 chitinase-oligosaccharide substrate interaction: subsite preference and anomer selectivity of Serratia marcescens chitinase A., Aronson NN Jr, Halloran BA, Alexyev MF, Amable L, Madura JD, Pasupulati L, Worth C, Van Roey P, Biochem J. 2003 Nov 15;376(Pt 1):87-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12932195 12932195]
-[[Category: Chitinase]]
 [[Category: Serratia marcescens]]
-[[Category: Single protein]]
+[[Category: Alexyev MF]]
-[[Category: Alexyev, M F.]]
+[[Category: Amable L]]
-[[Category: Amable, L.]]
+[[Category: Aronson Jr NN]]
-[[Category: Halloran, B A.]]
+[[Category: Halloran BA]]
-[[Category: Jr., N N.Aronson.]]
+[[Category: Madura JD]]
-[[Category: Madura, J D.]]
+[[Category: Pasupulati L]]
-[[Category: Pasupulati, L.]]
+[[Category: Van Roey P]]
-[[Category: Roey, P Van.]]
+[[Category: Worth C]]
-[[Category: Worth, C.]]
-[[Category: Oligosaccharide complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:50:07 2008''