1q0v: Difference between revisions

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-{{Seed}}
-[[Image:1q0v.png|left|200px]]
-<!--
+==Solution Structure of Tandem UIMs of Vps27==
-The line below this paragraph, containing "STRUCTURE_1q0v", creates the "Structure Box" on the page.
+<StructureSection load='1q0v' size='340' side='right'caption='[[1q0v]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1q0v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q0V FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q0v OCA], [https://pdbe.org/1q0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q0v RCSB], [https://www.ebi.ac.uk/pdbsum/1q0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q0v ProSAT]</span></td></tr>
-{{STRUCTURE_1q0v|  PDB=1q0v  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/VPS27_YEAST VPS27_YEAST] Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane. Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi. Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC. Might also function as an alternate adapter in the COPIb clathrin-like coat.<ref>PMID:3062374</ref> <ref>PMID:1493335</ref> <ref>PMID:8649377</ref> <ref>PMID:9015300</ref> <ref>PMID:9265642</ref> <ref>PMID:11208109</ref> <ref>PMID:11416128</ref> <ref>PMID:12055639</ref> <ref>PMID:11872141</ref> <ref>PMID:12900393</ref> <ref>PMID:14581452</ref> <ref>PMID:15166140</ref> <ref>PMID:15107463</ref> <ref>PMID:15086794</ref> <ref>PMID:17101773</ref> <ref>PMID:17135292</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q0/1q0v_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q0v ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Monoubiquitylation is a well-characterized signal for the internalization and sorting of integral membrane proteins to distinct cellular organelles. Recognition and transmission of monoubiquitin signals is mediated by a variety of ubiquitin-binding motifs such as UIM, UBA, UEV, VHS and CUE in endocytic proteins. The yeast Vps27 protein requires two UIMs for efficient interactions with ubiquitin and for sorting cargo into multivesicular bodies. Here we show that the individual UIMs of Vps27 exist as autonomously folded alpha-helices that bind ubiquitin independently, non-cooperatively and with modest affinity. The Vps27 N-terminal UIM engages the Leu8-Ile44-Val70 hydrophobic patch of ubiquitin through a helical surface conserved in UIMs of diverse proteins, including that of the S5a proteasomal regulatory subunit. The Leu8-Ile44-Val70 ubiquitin surface is also the site of interaction for CUE and UBA domains in endocytic proteins, consistent with the view that ubiquitin-binding endocytic proteins act serially on the same monoubiquitylated cargo during transport from cell surface to the lysosome.
-===Solution Structure of Tandem UIMs of Vps27===
+Solution structure of Vps27 UIM-ubiquitin complex important for endosomal sorting and receptor downregulation.,Swanson KA, Kang RS, Stamenova SD, Hicke L, Radhakrishnan I EMBO J. 2003 Sep 15;22(18):4597-606. PMID:12970172<ref>PMID:12970172</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_12970172}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1q0v" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 12970172 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_12970172}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-Q0V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q0V OCA].
-==Reference==
-Solution structure of Vps27 UIM-ubiquitin complex important for endosomal sorting and receptor downregulation., Swanson KA, Kang RS, Stamenova SD, Hicke L, Radhakrishnan I, EMBO J. 2003 Sep 15;22(18):4597-606. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12970172 12970172]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Single protein]]
+[[Category: Hicke L]]
-[[Category: Hicke, L.]]
+[[Category: Kang RS]]
-[[Category: Kang, R S.]]
+[[Category: Radhakrishnan I]]
-[[Category: Radhakrishnan, I.]]
+[[Category: Stamenova SD]]
-[[Category: Stamenova, S D.]]
+[[Category: Swanson KA]]
-[[Category: Swanson, K A.]]
-[[Category: Non-interacting alpha-helice]]
-[[Category: Stable]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:18:43 2008''