1jal: Difference between revisions

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New page: left|200px<br /><applet load="1jal" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jal, resolution 2.40Å" /> '''YCHF PROTEIN (HI0393...
 
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[[Image:1jal.gif|left|200px]]<br /><applet load="1jal" size="450" color="white" frame="true" align="right" spinBox="true"
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'''YCHF PROTEIN (HI0393)'''<br />


==Overview==
==YCHF PROTEIN (HI0393)==
The bacterial protein encoded by the gene ychF is 1 of 11 universally, conserved GTPases and the only one whose function is unknown. The crystal, structure determination of YchF was sought to help with the functional, assignment of the protein. The YchF protein from Haemophilus influenzae, was cloned and expressed, and the crystal structure was determined at 2.4, A resolution. The polypeptide chain is folded into three domains. The, N-terminal domain has a mononucleotide binding fold typical for the P-loop, NTPases. An 80-residue domain next to it has a pronounced alpha-helical, coiled coil. The C-terminal domain features a six-stranded half-barrel, that curves around an alpha-helix. The crablike three-domain structure of, YchF suggests the binding site for a double-stranded nucleic acid in the, cleft between the domains. The structure of the putative GTP-binding site, is consistent with the postulated guanine specificity of the protein., Fluorescence measurements have demonstrated the ability of YchF to bind a, double-stranded nucleic acid and GTP. Taken together with other, experimental data and genomic analysis, these results suggest that YchF, may be part of a nucleoprotein complex and may function as a GTP-dependent, translation factor.
<StructureSection load='1jal' size='340' side='right'caption='[[1jal]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1jal]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JAL FirstGlance]. <br>
1JAL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JAL OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jal OCA], [https://pdbe.org/1jal PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jal RCSB], [https://www.ebi.ac.uk/pdbsum/1jal PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jal ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of the YchF protein reveals binding sites for GTP and nucleic acid., Teplyakov A, Obmolova G, Chu SY, Toedt J, Eisenstein E, Howard AJ, Gilliland GL, J Bacteriol. 2003 Jul;185(14):4031-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12837776 12837776]
== Function ==
[https://www.uniprot.org/uniprot/YCHF_HAEIN YCHF_HAEIN] ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner (By similarity). Does not hydrolyze GTP.[HAMAP-Rule:MF_00944]<ref>PMID:17430889</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ja/1jal_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jal ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Gilliland, G.L.]]
[[Category: Gilliland GL]]
[[Category: S2F, Structure.2.Function.Project.]]
[[Category: Teplyakov A]]
[[Category: Teplyakov, A.]]
[[Category: nucleotide-binding fold]]
[[Category: s2f]]
[[Category: structural genomics]]
[[Category: structure 2 function project]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:03:18 2007''

Latest revision as of 10:38, 7 February 2024

YCHF PROTEIN (HI0393)YCHF PROTEIN (HI0393)

Structural highlights

1jal is a 2 chain structure with sequence from Haemophilus influenzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

YCHF_HAEIN ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner (By similarity). Does not hydrolyze GTP.[HAMAP-Rule:MF_00944][1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Koller-Eichhorn R, Marquardt T, Gail R, Wittinghofer A, Kostrewa D, Kutay U, Kambach C. Human OLA1 defines an ATPase subfamily in the Obg family of GTP-binding proteins. J Biol Chem. 2007 Jul 6;282(27):19928-37. Epub 2007 Apr 12. PMID:17430889 doi:http://dx.doi.org/10.1074/jbc.M700541200

1jal, resolution 2.40Å

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