1idf: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1idf.jpg|left|200px]]<br /><applet load="1idf" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1idf, resolution 2.5&Aring;" />
-'''ISOCITRATE DEHYDROGENASE K230M MUTANT APO ENZYME'''<br />
-==Overview==
+==ISOCITRATE DEHYDROGENASE K230M MUTANT APO ENZYME==
-Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution, were used to solve the structures of two sequential intermediates formed, during the catalytic actions of isocitrate dehydrogenase. Both, intermediates are distinct from the enzyme-substrate and enzyme-product, complexes. Mutation of key catalytic residues changed the rate determining, steps so that protein and substrate intermediates within the overall, reaction pathway could be visualized.
+<StructureSection load='1idf' size='340' side='right'caption='[[1idf]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1idf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IDF FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1idf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idf OCA], [https://pdbe.org/1idf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1idf RCSB], [https://www.ebi.ac.uk/pdbsum/1idf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1idf ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IDH_ECOLI IDH_ECOLI]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/id/1idf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1idf ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-IDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IDF OCA].
+*[[Isocitrate dehydrogenase 3D structures|Isocitrate dehydrogenase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase., Bolduc JM, Dyer DH, Scott WG, Singer P, Sweet RM, Koshland DE Jr, Stoddard BL, Science. 1995 Jun 2;268(5215):1312-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7761851 7761851]
 [[Category: Escherichia coli]]
-[[Category: Isocitrate dehydrogenase (NADP(+))]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Bolduc JM]]
-[[Category: Bolduc, J.M.]]
+[[Category: Dyer DH]]
-[[Category: Dyer, D.H.]]
+[[Category: Koshland Junior DE]]
-[[Category: Junior, D.E.Koshland.]]
+[[Category: Scott WG]]
-[[Category: Scott, W.G.]]
+[[Category: Singer P]]
-[[Category: Singer, P.]]
+[[Category: Stoddard BL]]
-[[Category: Stoddard, B.L.]]
+[[Category: Sweet RM]]
-[[Category: Sweet, R.M.]]
-[[Category: oxidoreductase (nad(a)-choh(d))]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:18:42 2007''