1hqc: Difference between revisions

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New page: left|200px<br /><applet load="1hqc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hqc, resolution 3.2Å" /> '''STRUCTURE OF RUVB FRO...
 
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[[Image:1hqc.gif|left|200px]]<br /><applet load="1hqc" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1hqc, resolution 3.2&Aring;" />
'''STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8'''<br />


==Overview==
==STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8==
We report here the crystal structure of the RuvB motor protein from, Thermus thermophilus HB8, which drives branch migration of the Holliday, junction during homologous recombination. RuvB has a crescent-like, architecture consisting of three consecutive domains, the first two of, which are involved in ATP binding and hydrolysis. DNA is likely to, interact with a large basic cleft, which encompasses the ATP-binding, pocket and domain boundaries, whereas the junction-recognition protein, RuvA may bind a flexible beta-hairpin protruding from the N-terminal, domain. The structures of two subunits, related by a noncrystallographic, pseudo-2-fold axis, imply that conformational changes of motor protein, coupled with ATP hydrolysis may reflect motility essential for its, translocation around double-stranded DNA.
<StructureSection load='1hqc' size='340' side='right'caption='[[1hqc]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1hqc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HQC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hqc OCA], [https://pdbe.org/1hqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hqc RCSB], [https://www.ebi.ac.uk/pdbsum/1hqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hqc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RUVB_THET8 RUVB_THET8] The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg(2+)-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone.<ref>PMID:10485292</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/1hqc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hqc ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1HQC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MG and ADE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HQC OCA].
*[[Helicase 3D structures|Helicase 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8., Yamada K, Kunishima N, Mayanagi K, Ohnishi T, Nishino T, Iwasaki H, Shinagawa H, Morikawa K, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1442-7. Epub 2001 Feb 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11171970 11171970]
__TOC__
[[Category: Adenosinetriphosphatase]]
</StructureSection>
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: Iwasaki, H.]]
[[Category: Iwasaki H]]
[[Category: Kunishima, N.]]
[[Category: Kunishima N]]
[[Category: Mayanagi, K.]]
[[Category: Mayanagi K]]
[[Category: Morikawa, K.]]
[[Category: Morikawa K]]
[[Category: Yamada, K.]]
[[Category: Yamada K]]
[[Category: ADE]]
[[Category: MG]]
[[Category: complex with nucleotide]]
[[Category: extended aaa-atpase domain]]
[[Category: ruvb]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:44:36 2007''

Latest revision as of 16:23, 13 March 2024

STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8

Structural highlights

1hqc is a 2 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RUVB_THET8 The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg(2+)-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Yamada K, Fukuoh A, Iwasaki H, Shinagawa H. Novel properties of the Thermus thermophilus RuvB protein, which promotes branch migration of Holliday junctions. Mol Gen Genet. 1999 Jul;261(6):1001-11. PMID:10485292

1hqc, resolution 3.20Å

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