1god: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1god.gif|left|200px]]<br /><applet load="1god" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1god, resolution 2.8&Aring;" />
-'''MONOMERIC LYS-49 PHOSPHOLIPASE A2 HOMOLOGUE ISOLATED FROM THE VENOM OF CERROPHIDION (BOTHROPS) GODMANI'''<br />
-==Overview==
+==MONOMERIC LYS-49 PHOSPHOLIPASE A2 HOMOLOGUE ISOLATED FROM THE VENOM OF CERROPHIDION (BOTHROPS) GODMANI==
-Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a, Ca2+-independent mechanism which does not involve catalytic activity. With, the aim of determining the structural basis for this novel activity, we, have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated, from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 A, resolution by molecular replacement. The final model has been refined to a, final crystallografic residual (Rfactor) of 18.8% (Rfree = 28.2%), with, excellent stereochemistry. godMT-II is also monomeric in the crystalline, state, and small-angle X-ray scattering results demonstrate that the, protein is monomeric in solution under fisicochemical conditions similar, to those used in the crystallographic studies.
+<StructureSection load='1god' size='340' side='right'caption='[[1god]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1god]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cerrophidion_godmani Cerrophidion godmani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GOD FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1god FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1god OCA], [https://pdbe.org/1god PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1god RCSB], [https://www.ebi.ac.uk/pdbsum/1god PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1god ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PA2H2_CERGO PA2H2_CERGO] Snake venom phospholipase A2 (PLA2) that lacks enzymatic activity and shows myotoxicity.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/1god_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1god ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. With the aim of determining the structural basis for this novel activity, we have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 A resolution by molecular replacement. The final model has been refined to a final crystallografic residual (Rfactor) of 18.8% (Rfree = 28.2%), with excellent stereochemistry. godMT-II is also monomeric in the crystalline state, and small-angle X-ray scattering results demonstrate that the protein is monomeric in solution under fisicochemical conditions similar to those used in the crystallographic studies.
-==About this Structure==
+Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani.,Arni RK, Fontes MR, Barberato C, Gutierrez JM, Diaz C, Ward RJ Arch Biochem Biophys. 1999 Jun 15;366(2):177-82. PMID:10356281<ref>PMID:10356281</ref>
-GOD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cerrophidion_godmani Cerrophidion godmani]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GOD OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani., Arni RK, Fontes MR, Barberato C, Gutierrez JM, Diaz C, Ward RJ, Arch Biochem Biophys. 1999 Jun 15;366(2):177-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10356281 10356281]
+</div>
+<div class="pdbe-citations 1god" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Cerrophidion godmani]]
-[[Category: Phospholipase A(2)]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Arni RK]]
-[[Category: Arni, R.K.]]
+[[Category: Barberato C]]
-[[Category: Barberato, C.]]
+[[Category: Diaz-Oreiro C]]
-[[Category: Diaz-Oreiro, C.]]
+[[Category: Fontes MRM]]
-[[Category: Fontes, M.R.M.]]
+[[Category: Gutierrez JM]]
-[[Category: Gutierrez, J.M.]]
+[[Category: Ward RJ]]
-[[Category: Ward, R.J.]]
-[[Category: bothrops]]
-[[Category: lys49-phospholipase a2]]
-[[Category: snake venom]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:12:07 2007''