1hl6: Difference between revisions

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-{{Seed}}
-[[Image:1hl6.png|left|200px]]
-<!--
+==A novel mode of RBD-protein recognition in the Y14-mago complex==
-The line below this paragraph, containing "STRUCTURE_1hl6", creates the "Structure Box" on the page.
+<StructureSection load='1hl6' size='340' side='right'caption='[[1hl6]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1hl6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HL6 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hl6 OCA], [https://pdbe.org/1hl6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hl6 RCSB], [https://www.ebi.ac.uk/pdbsum/1hl6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hl6 ProSAT]</span></td></tr>
-{{STRUCTURE_1hl6|  PDB=1hl6  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RBM8A_DROME RBM8A_DROME] Involved in mRNA quality control via the nonsense-mediated mRNA decay (NMD) pathway. Also involved in localization of osk (oskar) mRNA in the posterior pole of oocytes via its interaction with mago.<ref>PMID:11691839</ref> <ref>PMID:12730685</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/1hl6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hl6 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Y14 and Mago are conserved eukaryotic proteins that associate with spliced mRNAs in the nucleus and remain associated at exon junctions during and after nuclear export. In the cytoplasm, Y14 is involved in mRNA quality control via the nonsense-mediated mRNA decay (NMD) pathway and, together with Mago, is involved in localization of osk (oskar) mRNA. We have determined the crystal structure of the complex between Drosophila melanogaster Y14 and Mago at a resolution of 2.5 A. The structure reveals an atypical mode of protein-protein recognition mediated by an RNA-binding domain (RBD). Instead of binding RNA, the RBD of Y14 engages its RNP1 and RNP2 motifs to bind Mago. Using structure-guided mutagenesis, we show that Mago is also a component of the NMD pathway, and that its association with Y14 is essential for function. Heterodimerization creates a single structural platform that interacts with the NMD machinery via phylogenetically conserved residues.
-===A NOVEL MODE OF RBD-PROTEIN RECOGNITION IN THE Y14-MAGO COMPLEX===
+A novel mode of RBD-protein recognition in the Y14-Mago complex.,Fribourg S, Gatfield D, Izaurralde E, Conti E Nat Struct Biol. 2003 Jun;10(6):433-9. PMID:12730685<ref>PMID:12730685</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_12730685}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1hl6" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 12730685 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_12730685}}
+__TOC__
+</StructureSection>
-==About this Structure==
-HL6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL6 OCA].
-==Reference==
-A novel mode of RBD-protein recognition in the Y14-Mago complex., Fribourg S, Gatfield D, Izaurralde E, Conti E, Nat Struct Biol. 2003 Jun;10(6):433-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12730685 12730685]
 [[Category: Drosophila melanogaster]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Conti, E.]]
+[[Category: Conti E]]
-[[Category: Fribourg, S.]]
+[[Category: Fribourg S]]
-[[Category: Gatfield, D.]]
+[[Category: Gatfield D]]
-[[Category: Izaurralde, E.]]
+[[Category: Izaurralde E]]
-[[Category: Yao, W.]]
+[[Category: Yao W]]
-[[Category: Exon-exon junction]]
-[[Category: Nmd]]
-[[Category: Oskar]]
-[[Category: Rdb]]
-[[Category: Rnp]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 08:20:55 2008''