1hj8: Difference between revisions

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[[Image:1hj8.png|left|200px]]


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==1.00 AA Trypsin from Atlantic Salmon==
The line below this paragraph, containing "STRUCTURE_1hj8", creates the "Structure Box" on the page.
<StructureSection load='1hj8' size='340' side='right'caption='[[1hj8]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1hj8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJ8 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_1hj8|  PDB=1hj8  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hj8 OCA], [https://pdbe.org/1hj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hj8 RCSB], [https://www.ebi.ac.uk/pdbsum/1hj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hj8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TRY1_SALSA TRY1_SALSA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hj8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hj8 ConSurf].
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Radiation damage is an inherent problem in protein X-ray crystallography and the process has recently been shown to be highly specific, exhibiting features such as cleavage of disulfide bonds, decarboxylation of acidic residues, increase in atomic B factors and increase in unit-cell volume. Reported here are two trypsin structures at atomic resolution (1.00 and 0.95 A), the data for which were collected at a third-generation synchrotron (ESRF) at two different beamlines. Both trypsin structures exhibit broken disulfide bonds; in particular, the bond from Cys191 to Cys220 is very sensitive to synchrotron radiation. The data set collected at the most intense beamline (ID14-EH4) shows increased structural radiation damage in terms of lower occupancies for cysteine residues, more breakage in the six disulfide bonds and more alternate conformations. It appears that high intensity and not only the total X-ray dose is most harmful to protein crystals.


===1.00 AA TRYPSIN FROM ATLANTIC SALMON===
Atomic resolution structures of trypsin provide insight into structural radiation damage.,Leiros HK, McSweeney SM, Smalas AO Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):488-97. PMID:11264577<ref>PMID:11264577</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1hj8" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_11264577}}, adds the Publication Abstract to the page
*[[Trypsin 3D structures|Trypsin 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 11264577 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_11264577}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
1HJ8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJ8 OCA].
 
==Reference==
Atomic resolution structures of trypsin provide insight into structural radiation damage., Leiros HK, McSweeney SM, Smalas AO, Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):488-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11264577 11264577]
[[Category: Salmo salar]]
[[Category: Salmo salar]]
[[Category: Single protein]]
[[Category: Leiros H-KS]]
[[Category: Trypsin]]
[[Category: Mcsweeney SM]]
[[Category: Leiros, H K.S.]]
[[Category: Smalas AO]]
[[Category: Mcsweeney, S M.]]
[[Category: Smalas, A O.]]
[[Category: Atomic resolution]]
[[Category: Disulphide bond breakage]]
[[Category: Radiation damage]]
[[Category: Salmon]]
[[Category: Trypsin]]
 
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