1fw6: Difference between revisions

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OCA

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-[[Image:1fw6.gif|left|200px]]<br /><applet load="1fw6" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1fw6, resolution 2.70&Aring;" />
-'''CRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEX'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEX==
-The MutS protein initiates DNA mismatch repair by recognizing mispaired, and unpaired bases embedded in duplex DNA and activating endo- and, exonucleases to remove the mismatch. Members of the MutS family also, possess a conserved ATPase activity that belongs to the ATP binding, cassette (ABC) superfamily. Here we report the crystal structure of a, ternary complex of MutS-DNA-ADP and assays of initiation of mismatch, repair in conjunction with perturbation of the composite ATPase active, site by mutagenesis. These studies indicate that MutS has to bind both ATP, and the mismatch DNA simultaneously in order to activate the other, mismatch repair proteins. We propose that the MutS ATPase activity plays a, proofreading role in DNA mismatch repair, verification of mismatch, recognition, and authorization of repair.
+<StructureSection load='1fw6' size='340' side='right'caption='[[1fw6]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1fw6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FW6 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw6 OCA], [https://pdbe.org/1fw6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fw6 RCSB], [https://www.ebi.ac.uk/pdbsum/1fw6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fw6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MUTS_THEAQ MUTS_THEAQ] This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/1fw6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fw6 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-FW6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with MG, SO4 and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FW6 OCA].
+*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Composite active site of an ABC ATPase: MutS uses ATP to verify mismatch recognition and authorize DNA repair., Junop MS, Obmolova G, Rausch K, Hsieh P, Yang W, Mol Cell. 2001 Jan;7(1):1-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11172706 11172706]
+[[Category: Large Structures]]
-[[Category: Single protein]]
 [[Category: Thermus aquaticus]]
-[[Category: Hsieh, P.]]
+[[Category: Hsieh P]]
-[[Category: Junop, M.S.]]
+[[Category: Junop MS]]
-[[Category: Obmolova, G.]]
+[[Category: Obmolova G]]
-[[Category: Rausch, K.]]
+[[Category: Rausch K]]
-[[Category: Yang, W.]]
+[[Category: Yang W]]
-[[Category: ADP]]
-[[Category: MG]]
-[[Category: SO4]]
-[[Category: abc atpase]]
-[[Category: kinked dna]]
-[[Category: multi-domain structure]]
-[[Category: protein-dna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:21:52 2007''