1feh: Difference between revisions

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New page: left|200px<br /><applet load="1feh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1feh, resolution 1.8Å" /> '''FE-ONLY HYDROGENASE F...
 
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[[Image:1feh.gif|left|200px]]<br /><applet load="1feh" size="450" color="white" frame="true" align="right" spinBox="true"
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'''FE-ONLY HYDROGENASE FROM CLOSTRIDIUM PASTEURIANUM'''<br />


==Overview==
==FE-ONLY HYDROGENASE FROM CLOSTRIDIUM PASTEURIANUM==
A three-dimensional structure for the monomeric iron-containing, hydrogenase (CpI) from Clostridium pasteurianum was determined to 1.8, angstrom resolution by x-ray crystallography using multiwavelength, anomalous dispersion (MAD) phasing. CpI, an enzyme that catalyzes the, two-electron reduction of two protons to yield dihydrogen, was found to, contain 20 gram atoms of iron per mole of protein, arranged into five, distinct [Fe-S] clusters. The probable active-site cluster, previously, termed the H-cluster, was found to be an unexpected arrangement of six, iron atoms existing as a [4Fe-4S] cubane subcluster covalently bridged by, a cysteinate thiol to a [2Fe] subcluster. The iron atoms of the [2Fe], subcluster both exist with an octahedral coordination geometry and are, bridged to each other by three non-protein atoms, assigned as two sulfide, atoms and one carbonyl or cyanide molecule. This structure provides, insights into the mechanism of biological hydrogen activation and has, broader implications for [Fe-S] cluster structure and function in, biological systems.
<StructureSection load='1feh' size='340' side='right'caption='[[1feh]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1feh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. The March 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Hydrogenase''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_3 10.2210/rcsb_pdb/mom_2009_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FEH FirstGlance]. <br>
1FEH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum] with HC1, SF4 and FES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin_hydrogenase Ferredoxin hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.7.2 1.12.7.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FEH OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HC1:2+IRON/2+SULFUR/5+CARBONYL/2+WATER+INORGANIC+CLUSTER'>HC1</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1feh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1feh OCA], [https://pdbe.org/1feh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1feh RCSB], [https://www.ebi.ac.uk/pdbsum/1feh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1feh ProSAT]</span></td></tr>
X-ray crystal structure of the Fe-only hydrogenase (CpI) from Clostridium pasteurianum to 1.8 angstrom resolution., Peters JW, Lanzilotta WN, Lemon BJ, Seefeldt LC, Science. 1998 Dec 4;282(5395):1853-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9836629 9836629]
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHF1_CLOPA PHF1_CLOPA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fe/1feh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1feh ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Clostridium pasteurianum]]
[[Category: Clostridium pasteurianum]]
[[Category: Ferredoxin hydrogenase]]
[[Category: Hydrogenase]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Lanzilotta, W.N.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Lemon, B.J.]]
[[Category: Lanzilotta WN]]
[[Category: Peters, J.W.]]
[[Category: Lemon BJ]]
[[Category: Seefeldt, L.C.]]
[[Category: Peters JW]]
[[Category: FES]]
[[Category: Seefeldt LC]]
[[Category: HC1]]
[[Category: SF4]]
[[Category: oxidoreductase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:51:14 2007''

Latest revision as of 10:13, 7 February 2024

FE-ONLY HYDROGENASE FROM CLOSTRIDIUM PASTEURIANUMFE-ONLY HYDROGENASE FROM CLOSTRIDIUM PASTEURIANUM

Structural highlights

1feh is a 1 chain structure with sequence from Clostridium pasteurianum. The March 2009 RCSB PDB Molecule of the Month feature on Hydrogenase by David Goodsell is 10.2210/rcsb_pdb/mom_2009_3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PHF1_CLOPA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1feh, resolution 1.80Å

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