1eei: Difference between revisions

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-{{Seed}}
-[[Image:1eei.png|left|200px]]
-<!--
+==CHOLERA TOXIN B-PENTAMER COMPLEXED WITH METANITROPHENYL-ALPHA-D-GALACTOSE==
-The line below this paragraph, containing "STRUCTURE_1eei", creates the "Structure Box" on the page.
+<StructureSection load='1eei' size='340' side='right'caption='[[1eei]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1eei]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EEI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EEI FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAA:METANITROPHENYL-ALPHA-D-GALACTOSIDE'>GAA</scene></td></tr>
-{{STRUCTURE_1eei|  PDB=1eei  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eei OCA], [https://pdbe.org/1eei PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eei RCSB], [https://www.ebi.ac.uk/pdbsum/1eei PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eei ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q57193_VIBCL Q57193_VIBCL]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cholera toxin (CT) and the closely related heat-labile enterotoxin of Escherichia coli (LT) are responsible for numerous cases of diarrhea worldwide, leading to considerable morbidity and mortality. The B subunits of these heterohexameric AB(5) toxins form a pentameric arrangement which is responsible for binding to the receptor GM1 of the target epithelial cells of the host. Blocking these B pentamer-receptor interactions forms an avenue for therapeutic intervention. Here, the structural characterization of potential receptor-blocking compounds are described based on the previously identified inhibitor m-nitrophenyl-alpha-D-galactoside (MNPG). The structure of a CTB-MNPG complex confirms that the binding mode of this inhibitor is identical in the two homologous toxins CT and LT and is characterized by a glycosyl linkage geometry that leads to displacement of a well ordered water molecule near the amide group of Gly33 by the O1-substituent of MNPG. This glycosyl geometry is not maintained in the absence of a substituent that can displace this water, as shown by a complex of LTB with p-aminophenyl-alpha-D-galactoside (PAPG). New compounds were synthesized to investigate the feasibility of maintaining the favorable binding interactions exhibited by MNPG while gaining increased affinity through the addition of hydrophobic substituents complementary to either of two hydrophobic regions of the receptor-binding site. The structural characterization of complexes of LTB with two of these compounds, 3-benzylaminocarbonylphenyl-alpha-D-galactoside (BAPG) and 2-phenethyl-7-(2,3-dihydrophthalazine-1,4-dione)-alpha-D-galactoside (PEPG), demonstrates a partial success in this goal. Both compounds exhibit a mixture of binding modes, some of which are presumably influenced by the local packing environment at multiple crystallographically independent binding sites. The terminal phenyl ring of BAPG associates either with the phenyl group of Tyr12 or with the hydrophobic patch formed by Lys34 and Ile58. The latter interaction is also made by the terminal phenyl substituent of PEPG, despite a larger ring system linking the galactose moiety to the terminal phenyl. However, neither BAPG nor PEPG displaces the intended target water molecule. Both of the designed compounds exhibit increased affinity relative to the galactose and to PAPG notwithstanding the failure to displace a bound water, confirming that additional favorable hydrophobic interactions can be gained by extending the starting inhibitor by a hydrophobic tail. The insight gained from these structures should allow the design of additional candidate inhibitors that retain both the glycosyl geometry and water displacement exhibited by MNPG and the favorable hydrophobic interactions exhibited by BAPG and PEPG.
-===CHOLERA TOXIN B-PENTAMER COMPLEXED WITH METANITROPHENYL-ALPHA-D-GALACTOSE===
+Exploration of the GM1 receptor-binding site of heat-labile enterotoxin and cholera toxin by phenyl-ring-containing galactose derivatives.,Fan E, Merritt EA, Zhang Z, Pickens JC, Roach C, Ahn M, Hol WG Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):201-12. PMID:11173465<ref>PMID:11173465</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1eei" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_11173465}}, adds the Publication Abstract to the page
+*[[Cholera toxin 3D structures|Cholera toxin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 11173465 is the PubMed ID number.
+*[[User:David Solfiell/sandbox 1|User:David Solfiell/sandbox 1]]
--->
+== References ==
-{{ABSTRACT_PUBMED_11173465}}
+<references/>
+__TOC__
-==About this Structure==
+</StructureSection>
-EEI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EEI OCA].
+[[Category: Large Structures]]
-==Reference==
-Exploration of the GM1 receptor-binding site of heat-labile enterotoxin and cholera toxin by phenyl-ring-containing galactose derivatives., Fan E, Merritt EA, Zhang Z, Pickens JC, Roach C, Ahn M, Hol WG, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):201-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11173465 11173465]
-[[Category: Single protein]]
 [[Category: Vibrio cholerae]]
-[[Category: Hol, W G.J.]]
+[[Category: Hol WGJ]]
-[[Category: Merritt, E A.]]
+[[Category: Merritt EA]]
-[[Category: Toxin,enterotoxin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 00:34:14 2008''