1f6b: Difference between revisions

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New page: left|200px<br /><applet load="1f6b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f6b, resolution 1.70Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1f6b.gif|left|200px]]<br /><applet load="1f6b" size="450" color="white" frame="true" align="right" spinBox="true"
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'''CRYSTAL STRUCTURE OF SAR1-GDP COMPLEX'''<br />


==Overview==
==CRYSTAL STRUCTURE OF SAR1-GDP COMPLEX==
The Sar1 GTPase is an essential component of COPII vesicle coats involved, in export of cargo from the ER. We report the 1.7-A structure of Sar1 and, find that consistent with the sequence divergence of Sar1 from Arf family, GTPases, Sar1 is structurally distinct. In particular, we show that the, Sar1 NH2 terminus contains two regions: an NH2-terminal extension, containing an evolutionary conserved hydrophobic motif that facilitates, membrane recruitment and activation by the mammalian Sec12 guanine, nucleotide exchange factor, and an alpha1' amphipathic helix that, contributes to interaction with the Sec23/24 complex that is responsible, for cargo selection during ER export. We propose that the hydrophobic Sar1, NH2-terminal activation/recruitment motif, in conjunction with the alpha1', helix, mediates the initial steps in COPII coat assembly for export from, the ER.
<StructureSection load='1f6b' size='340' side='right'caption='[[1f6b]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1f6b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F6B FirstGlance]. <br>
1F6B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with MG, SO4 and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F6B OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f6b OCA], [https://pdbe.org/1f6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f6b RCSB], [https://www.ebi.ac.uk/pdbsum/1f6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f6b ProSAT]</span></td></tr>
Crystal structure of Sar1-GDP at 1.7 A resolution and the role of the NH2 terminus in ER export., Huang M, Weissman JT, Beraud-Dufour S, Luan P, Wang C, Chen W, Aridor M, Wilson IA, Balch WE, J Cell Biol. 2001 Dec 10;155(6):937-48. Epub 2001 Dec 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11739406 11739406]
</table>
== Function ==
[https://www.uniprot.org/uniprot/SAR1B_CRIGR SAR1B_CRIGR] Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.<ref>PMID:8138575</ref> <ref>PMID:11149932</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/1f6b_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f6b ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cricetulus griseus]]
[[Category: Cricetulus griseus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Balch, W.E.]]
[[Category: Balch WE]]
[[Category: Huang, M.]]
[[Category: Huang M]]
[[Category: Wilson, I.A.]]
[[Category: Wilson IA]]
[[Category: GDP]]
[[Category: MG]]
[[Category: SO4]]
[[Category: gtpases]]
[[Category: mg-containing complex]]
[[Category: n-terminal helix]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:39:07 2007''

Latest revision as of 10:11, 7 February 2024

CRYSTAL STRUCTURE OF SAR1-GDP COMPLEXCRYSTAL STRUCTURE OF SAR1-GDP COMPLEX

Structural highlights

1f6b is a 2 chain structure with sequence from Cricetulus griseus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SAR1B_CRIGR Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Kuge O, Dascher C, Orci L, Rowe T, Amherdt M, Plutner H, Ravazzola M, Tanigawa G, Rothman JE, Balch WE. Sar1 promotes vesicle budding from the endoplasmic reticulum but not Golgi compartments. J Cell Biol. 1994 Apr;125(1):51-65. PMID:8138575
  2. ↑ Aridor M, Fish KN, Bannykh S, Weissman J, Roberts TH, Lippincott-Schwartz J, Balch WE. The Sar1 GTPase coordinates biosynthetic cargo selection with endoplasmic reticulum export site assembly. J Cell Biol. 2001 Jan 8;152(1):213-29. PMID:11149932

1f6b, resolution 1.70Å

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