1eif: Difference between revisions

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New page: left|200px<br /><applet load="1eif" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eif, resolution 1.9Å" /> '''EUKARYOTIC TRANSLATIO...
 
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[[Image:1eif.gif|left|200px]]<br /><applet load="1eif" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1eif, resolution 1.9&Aring;" />
'''EUKARYOTIC TRANSLATION INITIATION FACTOR 5A FROM METHANOCOCCUS JANNASCHII'''<br />


==Overview==
==EUKARYOTIC TRANSLATION INITIATION FACTOR 5A FROM METHANOCOCCUS JANNASCHII==
Eukaryotic translation initiation factor 5A (eIF-5A) is a ubiquitous, protein found in all eukaryotic cells. The protein is closely associated, with cell proliferation in the G1-S stage of the cell cycle. Recent, findings show that the eIF-5A proteins are highly expressed in tumor cells, and act as a cofactor of the Rev protein in HIV-1-infected cells. The, mature eIF is the only protein known to have the unusual amino acid, hypusine, a post-translationally modified lysine. The crystal structure of, eIF-5A from Methanococcus jannaschii (MJ eIF-5A) has been determined at, 1.9 A and 1.8 A resolution in two crystal forms by using the multiple, isomorphous replacement method and the multiwavelength anomalous, diffraction method for the first crystal form and the molecular, replacement method for the second crystal form. The structure consists of, two folding domains, one of which is similar to the, oligonucleotide-binding domain found in the prokaryotic cold shock protein, and the translation initiation factor IF1 despite the absence of any, significant sequence similarities. The 12 highly conserved amino acid, residues found among eIF-5As include the hypusine site and form a long, protruding loop at one end of the elongated molecule.
<StructureSection load='1eif' size='340' side='right'caption='[[1eif]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1eif]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eif OCA], [https://pdbe.org/1eif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eif RCSB], [https://www.ebi.ac.uk/pdbsum/1eif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eif ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IF5A_METJA IF5A_METJA] Functions by promoting the formation of the first peptide bond.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1eif_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eif ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1EIF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EIF OCA].
*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structures of eukaryotic translation initiation factor 5A from Methanococcus jannaschii at 1.8 A resolution., Kim KK, Hung LW, Yokota H, Kim R, Kim SH, Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10419-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9724718 9724718]
[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Single protein]]
[[Category: Hung LW]]
[[Category: Hung, L.W.]]
[[Category: Kim KK]]
[[Category: Kim, K.K.]]
[[Category: Kim R]]
[[Category: Kim, R.]]
[[Category: Kim SH]]
[[Category: Kim, S.H.]]
[[Category: Yokota H]]
[[Category: Yokota, H.]]
[[Category: eif-5a]]
[[Category: initiation factor]]
[[Category: ob-fold]]
[[Category: translation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:01:27 2007''

Latest revision as of 10:03, 7 February 2024

EUKARYOTIC TRANSLATION INITIATION FACTOR 5A FROM METHANOCOCCUS JANNASCHIIEUKARYOTIC TRANSLATION INITIATION FACTOR 5A FROM METHANOCOCCUS JANNASCHII

Structural highlights

1eif is a 1 chain structure with sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IF5A_METJA Functions by promoting the formation of the first peptide bond.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1eif, resolution 1.90Å

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