1ae3: Difference between revisions

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[[Image:1ae3.png|left|200px]]


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==MUTANT R82C OF GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)==
The line below this paragraph, containing "STRUCTURE_1ae3", creates the "Structure Box" on the page.
<StructureSection load='1ae3' size='340' side='right'caption='[[1ae3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1ae3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE3 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae3 OCA], [https://pdbe.org/1ae3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae3 ProSAT]</span></td></tr>
{{STRUCTURE_1ae3| PDB=1ae3 |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/G5P_BPF1 G5P_BPF1] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae3_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae3 ConSurf].
<div style="clear:both"></div>


===MUTANT R82C OF GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)===
==See Also==
 
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
 
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</StructureSection>
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{{ABSTRACT_PUBMED_9098886}}
 
==About this Structure==
1AE3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE3 OCA].
 
==Reference==
Analyses of the stability and function of three surface mutants (R82C, K69H, and L32R) of the gene V protein from Ff phage by X-ray crystallography., Su S, Gao YG, Zhang H, Terwilliger TC, Wang AH, Protein Sci. 1997 Apr;6(4):771-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9098886 9098886]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Gao, Y G.]]
[[Category: Gao Y-G]]
[[Category: Su, S.]]
[[Category: Su S]]
[[Category: Terwilliger, T C.]]
[[Category: Terwilliger TC]]
[[Category: Wang, A H.J.]]
[[Category: Wang AH-J]]
[[Category: Zhang, H.]]
[[Category: Zhang H]]
[[Category: Dna replication]]
[[Category: Gvp]]
[[Category: Mutant]]
[[Category: R82c]]
[[Category: Single-stranded dna binding protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:39:17 2008''

Latest revision as of 09:29, 7 February 2024

MUTANT R82C OF GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)MUTANT R82C OF GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)

Structural highlights

1ae3 is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G5P_BPF1 Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1ae3, resolution 2.00Å

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