1d2e: Difference between revisions

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New page: left|200px<br /><applet load="1d2e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d2e, resolution 1.94Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1d2e.jpg|left|200px]]<br /><applet load="1d2e" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1d2e, resolution 1.94&Aring;" />
'''CRYSTAL STRUCTURE OF MITOCHONDRIAL EF-TU IN COMPLEX WITH GDP'''<br />


==Overview==
==CRYSTAL STRUCTURE OF MITOCHONDRIAL EF-TU IN COMPLEX WITH GDP==
The crystal structure of bovine mitochondrial elongation factor Tu (EF-Tu), in complex with GDP has been determined at a resolution of 1. 94 A. The, structure is similar to that of EF-Tu:GDP from Escherichia coli and, Thermus aquaticus, but the orientation of the GDP-binding domain 1 is, changed relative to domains 2 and 3. Sixteen conserved water molecules, common to EF-Tu and other G-proteins in the GDP-binding site are, described. These water molecules create a network linking separated parts, of the binding pocket. Mitochondrial EF-Tu binds nucleotides less tightly, than prokaryotic EF-Tu possibly due to an increased mobility in regions, close to the GDP-binding site. The C-terminal extension of mitochondrial, EF-Tu has structural similarities with DNA recognising zinc fingers, suggesting that the extension may be involved in recognition of RNA.
<StructureSection load='1d2e' size='340' side='right'caption='[[1d2e]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1d2e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D2E FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d2e OCA], [https://pdbe.org/1d2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d2e RCSB], [https://www.ebi.ac.uk/pdbsum/1d2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d2e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/EFTU_BOVIN EFTU_BOVIN] This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d2/1d2e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d2e ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1D2E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D2E OCA].
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
High resolution crystal structure of bovine mitochondrial EF-Tu in complex with GDP., Andersen GR, Thirup S, Spremulli LL, Nyborg J, J Mol Biol. 2000 Mar 24;297(2):421-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10715211 10715211]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Andersen, G.R.]]
[[Category: Andersen GR]]
[[Category: Nyborg, J.]]
[[Category: Nyborg J]]
[[Category: Spremulli, L.L.]]
[[Category: Spremulli LL]]
[[Category: Thirup, S.]]
[[Category: Thirup S]]
[[Category: GDP]]
[[Category: MG]]
[[Category: beta-barrel]]
[[Category: g-protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:57:28 2007''

Latest revision as of 09:48, 7 February 2024

CRYSTAL STRUCTURE OF MITOCHONDRIAL EF-TU IN COMPLEX WITH GDPCRYSTAL STRUCTURE OF MITOCHONDRIAL EF-TU IN COMPLEX WITH GDP

Structural highlights

1d2e is a 4 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.94Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EFTU_BOVIN This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1d2e, resolution 1.94Å

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