1crb: Difference between revisions

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New page: left|200px<br /><applet load="1crb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1crb, resolution 2.1Å" /> '''CRYSTALLOGRAPHIC STUD...
 
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[[Image:1crb.gif|left|200px]]<br /><applet load="1crb" size="450" color="white" frame="true" align="right" spinBox="true"
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'''CRYSTALLOGRAPHIC STUDIES ON A FAMILY OF CELLULAR LIPOPHILIC TRANSPORT PROTEINS. REFINEMENT OF P2 MYELIN PROTEIN AND THE STRUCTURE DETERMINATION AND REFINEMENT OF CELLULAR RETINOL-BINDING PROTEIN IN COMPLEX WITH ALL-TRANS-RETINOL'''<br />


==Overview==
==CRYSTALLOGRAPHIC STUDIES ON A FAMILY OF CELLULAR LIPOPHILIC TRANSPORT PROTEINS. REFINEMENT OF P2 MYELIN PROTEIN AND THE STRUCTURE DETERMINATION AND REFINEMENT OF CELLULAR RETINOL-BINDING PROTEIN IN COMPLEX WITH ALL-TRANS-RETINOL==
P2 myelin protein (P2) and cellular retinol binding protein (CRBP) are, members of a family of cellular lipophilic transport proteins. P2 has been, refined at a resolution of 2.7 A, and CRBP has been solved by molecular, replacement and refined to a resolution of 2.1 A. The members of this, family form a compact three-dimensional structure built up from ten, antiparallel strands that fold to form an orthogonal barrel containing the, ligand. In P2, the carboxylate group of an oleic acid ligand interacts, with the side-chains of two arginine (106 and 126), and one tyrosine (128), residues. The ligand adopts a U-shaped conformation. In CRBP, the, all-trans-retinol has a planar conformation with its alcohol group, hydrogen bonding to the side-chain of glutamine 108 (equivalent to residue, 106 in P2). The local interactions of glutamine 108 explain CRBP's, preference for binding retinol rather than retinal. The side-chain of, lysine 40 makes a close contact with the isoprene tail of the retinol.
<StructureSection load='1crb' size='340' side='right'caption='[[1crb]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1crb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CRB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=RTL:RETINOL'>RTL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1crb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crb OCA], [https://pdbe.org/1crb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1crb RCSB], [https://www.ebi.ac.uk/pdbsum/1crb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1crb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RET1_RAT RET1_RAT] Intracellular transport of retinol.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/1crb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1crb ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1CRB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with CD and RTL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CRB OCA].
*[[Retinol-binding protein 3D structures|Retinol-binding protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystallographic studies on a family of cellular lipophilic transport proteins. Refinement of P2 myelin protein and the structure determination and refinement of cellular retinol-binding protein in complex with all-trans-retinol., Cowan SW, Newcomer ME, Jones TA, J Mol Biol. 1993 Apr 20;230(4):1225-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7683727 7683727]
[[Category: Large Structures]]
[[Category: Rattus rattus]]
[[Category: Rattus rattus]]
[[Category: Single protein]]
[[Category: Cowan SW]]
[[Category: Cowan, S.W.]]
[[Category: Jones TA]]
[[Category: Jones, T.A.]]
[[Category: CD]]
[[Category: RTL]]
[[Category: cellular lipophilic transport protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:41:52 2007''

Latest revision as of 09:45, 7 February 2024

CRYSTALLOGRAPHIC STUDIES ON A FAMILY OF CELLULAR LIPOPHILIC TRANSPORT PROTEINS. REFINEMENT OF P2 MYELIN PROTEIN AND THE STRUCTURE DETERMINATION AND REFINEMENT OF CELLULAR RETINOL-BINDING PROTEIN IN COMPLEX WITH ALL-TRANS-RETINOLCRYSTALLOGRAPHIC STUDIES ON A FAMILY OF CELLULAR LIPOPHILIC TRANSPORT PROTEINS. REFINEMENT OF P2 MYELIN PROTEIN AND THE STRUCTURE DETERMINATION AND REFINEMENT OF CELLULAR RETINOL-BINDING PROTEIN IN COMPLEX WITH ALL-TRANS-RETINOL

Structural highlights

1crb is a 1 chain structure with sequence from Rattus rattus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RET1_RAT Intracellular transport of retinol.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1crb, resolution 2.10Å

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