1b4b: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1b4b.jpg|left|200px]]<br /><applet load="1b4b" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1b4b, resolution 2.2&Aring;" />
-'''STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF THE ARGININE REPRESSOR FROM BACILLUS STEAROTHERMOPHILUS'''<br />
-==Overview==
+==STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF THE ARGININE REPRESSOR FROM BACILLUS STEAROTHERMOPHILUS==
-The arginine repressor (ArgR) is a hexameric DNA-binding protein that, plays a multifunctional role in the bacterial cell. Here, we present the, 2.5 A structure of apo-ArgR from Bacillus stearothermophilus and the 2.2 A, structure of the hexameric ArgR oligomerization domain with bound, arginine. This first view of intact ArgR reveals an approximately, 32-symmetric hexamer of identical subunits, with six DNA-binding domains, surrounding a central oligomeric core. The difference in quaternary, organization of subunits in the arginine-bound and apo forms provides a, possible explanation for poor operator binding by apo-ArgR and for high, affinity binding in the presence of arginine.
+<StructureSection load='1b4b' size='340' side='right'caption='[[1b4b]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1b4b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B4B FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b4b OCA], [https://pdbe.org/1b4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b4b RCSB], [https://www.ebi.ac.uk/pdbsum/1b4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b4b ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ARGR_GEOSE ARGR_GEOSE] Regulates arginine biosynthesis genes.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b4/1b4b_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b4b ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The arginine repressor (ArgR) is a hexameric DNA-binding protein that plays a multifunctional role in the bacterial cell. Here, we present the 2.5 A structure of apo-ArgR from Bacillus stearothermophilus and the 2.2 A structure of the hexameric ArgR oligomerization domain with bound arginine. This first view of intact ArgR reveals an approximately 32-symmetric hexamer of identical subunits, with six DNA-binding domains surrounding a central oligomeric core. The difference in quaternary organization of subunits in the arginine-bound and apo forms provides a possible explanation for poor operator binding by apo-ArgR and for high affinity binding in the presence of arginine.
-==About this Structure==
+Structure of the arginine repressor from Bacillus stearothermophilus.,Ni J, Sakanyan V, Charlier D, Glansdorff N, Van Duyne GD Nat Struct Biol. 1999 May;6(5):427-32. PMID:10331868<ref>PMID:10331868</ref>
-B4B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with ARG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B4B OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Structure of the arginine repressor from Bacillus stearothermophilus., Ni J, Sakanyan V, Charlier D, Glansdorff N, Van Duyne GD, Nat Struct Biol. 1999 May;6(5):427-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10331868 10331868]
+</div>
+<div class="pdbe-citations 1b4b" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Arginine repressor 3D structures|Arginine repressor 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Geobacillus stearothermophilus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Charlier, D.]]
+[[Category: Charlier D]]
-[[Category: Duyne, G.D.Van.]]
+[[Category: Glansdorff N]]
-[[Category: Glansdorff, N.]]
+[[Category: Ni J]]
-[[Category: Ni, J.]]
+[[Category: Sakanyan V]]
-[[Category: Sakanyan, V.]]
+[[Category: Van Duyne GD]]
-[[Category: ARG]]
-[[Category: arginine]]
-[[Category: core]]
-[[Category: helix turn helix]]
-[[Category: oligomerization domain]]
-[[Category: repressor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:20:54 2007''