1a8y: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /><applet load="1a8y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a8y, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ...
 
No edit summary
 
(16 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1a8y.gif|left|200px]]<br /><applet load="1a8y" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1a8y, resolution 2.4&Aring;" />
'''CRYSTAL STRUCTURE OF CALSEQUESTRIN FROM RABBIT SKELETAL MUSCLE SARCOPLASMIC RETICULUM AT 2.4 A RESOLUTION'''<br />


==Overview==
==CRYSTAL STRUCTURE OF CALSEQUESTRIN FROM RABBIT SKELETAL MUSCLE SARCOPLASMIC RETICULUM AT 2.4 A RESOLUTION==
Calsequestrin, the major Ca2+ storage protein of muscle, coordinately, binds and releases 40-50 Ca2+ ions per molecule for each, contraction-relaxation cycle by an uncertain mechanism. We have determined, the structure of rabbit skeletal muscle calsequestrin. Three very negative, thioredoxin-like domains surround a hydrophilic center. Each monomer makes, two extensive dimerization contacts, both of which involve the approach of, many negative groups. This structure suggests a mechanism by which, calsequestrin may achieve high capacity Ca2+ binding. The suggested, mechanism involves Ca2+-induced collapse of the three domains and, polymerization of calsequestrin monomers arising from three factors:, N-terminal arm exchange, helix-helix contacts and Ca2+ cross bridges. This, proposed structure-based mechanism accounts for the observed coupling of, high capacity Ca2+ binding with protein precipitation.
<StructureSection load='1a8y' size='340' side='right'caption='[[1a8y]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1a8y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A8Y FirstGlance]. <br>
1A8Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A8Y OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a8y OCA], [https://pdbe.org/1a8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a8y RCSB], [https://www.ebi.ac.uk/pdbsum/1a8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a8y ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of calsequestrin from rabbit skeletal muscle sarcoplasmic reticulum., Wang S, Trumble WR, Liao H, Wesson CR, Dunker AK, Kang CH, Nat Struct Biol. 1998 Jun;5(6):476-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9628486 9628486]
== Function ==
[https://www.uniprot.org/uniprot/CASQ1_RABIT CASQ1_RABIT] Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+) ions (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/1a8y_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a8y ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
[[Category: Single protein]]
[[Category: Dunker AK]]
[[Category: Dunker, A.K.]]
[[Category: Kang C]]
[[Category: Kang, C.]]
[[Category: Liao H]]
[[Category: Liao, H.]]
[[Category: Trumble WR]]
[[Category: Trumble, W.R.]]
[[Category: Wang S]]
[[Category: Wang, S.]]
[[Category: Wesson CR]]
[[Category: Wesson, C.R.]]
[[Category: calcium-binding protein]]
[[Category: calsequestrin]]
[[Category: rabbit skeletal muscle]]
[[Category: sarcoplasmic reticulum]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:41:17 2007''

Latest revision as of 09:28, 7 February 2024

CRYSTAL STRUCTURE OF CALSEQUESTRIN FROM RABBIT SKELETAL MUSCLE SARCOPLASMIC RETICULUM AT 2.4 A RESOLUTIONCRYSTAL STRUCTURE OF CALSEQUESTRIN FROM RABBIT SKELETAL MUSCLE SARCOPLASMIC RETICULUM AT 2.4 A RESOLUTION

Structural highlights

1a8y is a 1 chain structure with sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CASQ1_RABIT Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+) ions (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1a8y, resolution 2.40Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1a8y&oldid=4039982"