1ad0: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1ad0.gif|left|200px]]<br />
-<applet load="1ad0" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1ad0, resolution 2.50&Aring;" />
-'''FAB FRAGMENT OF ENGINEERED HUMAN MONOCLONAL ANTIBODY A5B7'''<br />
-==Overview==
+==FAB FRAGMENT OF ENGINEERED HUMAN MONOCLONAL ANTIBODY A5B7==
-The crystal structures of two pairs of Fab fragments have been determined., The pairs comprise both a murine and an engineered human form, each, derived from the antitumor antibodies A5B7 and CTM01. Although antigen, specificity is maintained within the pairs, antigen affinity varies. A, comparison of the hypervariable loops for each pair of antibodies shows, their structure has been well maintained in grafting, supporting the, canonical loop model. Detailed structural analysis of the binding sites, and domain arrangements for these antibodies suggests the differences in, antigen affinity observed are likely to be due to inherent flexibility of, the hypervariable loops and movements at the VL:VH domain interface. The, four structures provide the first opportunity to study in detail the, effects of protein engineering on specific antibodies.
+<StructureSection load='1ad0' size='340' side='right'caption='[[1ad0]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ad0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AD0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ad0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ad0 OCA], [https://pdbe.org/1ad0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ad0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ad0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ad0 ProSAT]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ad/1ad0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ad0 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The crystal structures of two pairs of Fab fragments have been determined. The pairs comprise both a murine and an engineered human form, each derived from the antitumor antibodies A5B7 and CTM01. Although antigen specificity is maintained within the pairs, antigen affinity varies. A comparison of the hypervariable loops for each pair of antibodies shows their structure has been well maintained in grafting, supporting the canonical loop model. Detailed structural analysis of the binding sites and domain arrangements for these antibodies suggests the differences in antigen affinity observed are likely to be due to inherent flexibility of the hypervariable loops and movements at the VL:VH domain interface. The four structures provide the first opportunity to study in detail the effects of protein engineering on specific antibodies.
-==About this Structure==
+VL:VH domain rotations in engineered antibodies: crystal structures of the Fab fragments from two murine antitumor antibodies and their engineered human constructs.,Banfield MJ, King DJ, Mountain A, Brady RL Proteins. 1997 Oct;29(2):161-71. PMID:9329081<ref>PMID:9329081</ref>
-AD0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AD0 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-VL:VH domain rotations in engineered antibodies: crystal structures of the Fab fragments from two murine antitumor antibodies and their engineered human constructs., Banfield MJ, King DJ, Mountain A, Brady RL, Proteins. 1997 Oct;29(2):161-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9329081 9329081]
+</div>
+<div class="pdbe-citations 1ad0" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Antibody 3D structures|Antibody 3D structures]]
+*[[Sandbox 20009|Sandbox 20009]]
+*[[3D structures of human antibody|3D structures of human antibody]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Banfield, M.J.]]
+[[Category: Banfield MJ]]
-[[Category: Brady, R.L.]]
+[[Category: Brady RL]]
-[[Category: fab fragment]]
-[[Category: immunoglobulin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:25:51 2007''