1brl: Difference between revisions

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New page: left|200px<br /> <applet load="1brl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1brl, resolution 2.4Å" /> '''THREE-DIMENSIONAL ST...
 
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[[Image:1brl.gif|left|200px]]<br />
<applet load="1brl" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1brl, resolution 2.4&Aring;" />
'''THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION'''<br />


==Overview==
==THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION==
Luciferases are a class of enzymes that generate light in the visible, spectrum. Luciferase from luminous marine bacteria is an alpha-beta, heterodimer monooxygenase that catalyzes the oxidation of FMNH2 and a, long-chain aliphatic aldehyde. The X-ray crystal structure of bacterial, luciferase from Vibrio harveyi has been determined to 2.4 A resolution., The structure was solved by a combination of multiple isomorphous, replacement and molecular averaging between the two heterodimers in the, asymmetric unit. Each subunit folds into a (beta/alpha)8 barrel motif, and, dimerization is mediated through a parallel four-helix bundle centered on, a pseudo 2-fold axis that relates the structurally similar subunits. The, vicinity of the active site has been identified on the alpha subunit by, correlations with similar protein motifs and previous biochemical studies., The structure presented here represents the first molecular model of a, bioluminescent enzyme.
<StructureSection load='1brl' size='340' side='right'caption='[[1brl]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1brl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. The June 2006 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Luciferase''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2006_6 10.2210/rcsb_pdb/mom_2006_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BRL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BRL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1brl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1brl OCA], [https://pdbe.org/1brl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1brl RCSB], [https://www.ebi.ac.uk/pdbsum/1brl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1brl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LUXA_VIBHA LUXA_VIBHA] Light-emitting reaction in luminous bacteria.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/1brl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1brl ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1BRL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1BRL with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb78_1.html Luciferase]]. Active as [http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BRL OCA].
*[[Luciferase 3D structures|Luciferase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution., Fisher AJ, Raushel FM, Baldwin TO, Rayment I, Biochemistry. 1995 May 23;34(20):6581-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7756289 7756289]
[[Category: Large Structures]]
[[Category: Alkanal monooxygenase (FMN-linked)]]
[[Category: Luciferase]]
[[Category: Luciferase]]
[[Category: Protein complex]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Vibrio harveyi]]
[[Category: Vibrio harveyi]]
[[Category: Fisher, A.J.]]
[[Category: Fisher AJ]]
[[Category: Rayment, I.]]
[[Category: Rayment I]]
[[Category: PO4]]
[[Category: monooxygenase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 08:57:51 2007''

Latest revision as of 09:38, 7 February 2024

THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTIONTHREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION

Structural highlights

1brl is a 4 chain structure with sequence from Vibrio harveyi. The June 2006 RCSB PDB Molecule of the Month feature on Luciferase by David S. Goodsell is 10.2210/rcsb_pdb/mom_2006_6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LUXA_VIBHA Light-emitting reaction in luminous bacteria.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1brl, resolution 2.40Å

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