2i2y: Difference between revisions

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-[[Image:2i2y.gif|left|200px]]
-<!--
-The line below this paragraph, containing "STRUCTURE_2i2y", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
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-{{STRUCTURE_2i2y|  PDB=2i2y  |  SCENE=  }}
-'''Solution structure of the RRM of SRp20 bound to the RNA CAUC'''
+==Solution structure of the RRM of SRp20 bound to the RNA CAUC==
+<StructureSection load='2i2y' size='340' side='right'caption='[[2i2y]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2i2y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G']. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I2Y FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2y OCA], [https://pdbe.org/2i2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i2y RCSB], [https://www.ebi.ac.uk/pdbsum/2i2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i2y ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPG2_STRSG SPG2_STRSG] [https://www.uniprot.org/uniprot/SRSF3_HUMAN SRSF3_HUMAN] Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing (PubMed:17036044, PubMed:26876937, PubMed:32440474). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons (PubMed:26876937). Also functions as an adapter involved in mRNA nuclear export (PubMed:11336712, PubMed:18364396, PubMed:28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed:11336712, PubMed:18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244).<ref>PMID:11336712</ref> <ref>PMID:17036044</ref> <ref>PMID:18364396</ref> <ref>PMID:26876937</ref> <ref>PMID:28984244</ref> <ref>PMID:32440474</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i2/2i2y_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i2y ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest members of the serine- and arginine-rich (SR) protein family, well known for their role in splicing. They also play a role in mRNA export, in particular of histone mRNAs. We present the solution structures of the free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals that although all 4 nt are contacted by the RRM, only the 5' cytosine is primarily recognized in a specific way. This might explain the numerous consensus sequences found by SELEX (systematic evolution of ligands by exponential enrichment) for the RRM of 9G8 and SRp20. Furthermore, we identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction with the export factor Tip-associated protein (TAP). Together, these results provide a molecular description for mRNA and TAP recognition by SRp20 and 9G8.
-==Overview==
+Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.,Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH EMBO J. 2006 Nov 1;25(21):5126-37. Epub 2006 Oct 12. PMID:17036044<ref>PMID:17036044</ref>
-The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest members of the serine- and arginine-rich (SR) protein family, well known for their role in splicing. They also play a role in mRNA export, in particular of histone mRNAs. We present the solution structures of the free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals that although all 4 nt are contacted by the RRM, only the 5' cytosine is primarily recognized in a specific way. This might explain the numerous consensus sequences found by SELEX (systematic evolution of ligands by exponential enrichment) for the RRM of 9G8 and SRp20. Furthermore, we identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction with the export factor Tip-associated protein (TAP). Together, these results provide a molecular description for mRNA and TAP recognition by SRp20 and 9G8.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-I2Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._group_g_and_homo_sapiens Streptococcus sp. group g and homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2Y OCA].
+</div>
+<div class="pdbe-citations 2i2y" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8., Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH, EMBO J. 2006 Nov 1;25(21):5126-37. Epub 2006 Oct 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17036044 17036044]
+*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
-[[Category: Single protein]]
+== References ==
-[[Category: Streptococcus sp. group g and homo sapiens]]
+<references/>
-[[Category: Allain, F H.]]
+__TOC__
-[[Category: Hargous, Y F.]]
+</StructureSection>
-[[Category: Protein-rna complex rrm alpha-beta sandwich beta1-alpha1-beta2-beta3-alpha2-beta4]]
+[[Category: Homo sapiens]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:00:32 2008''
+[[Category: Large Structures]]
+[[Category: Streptococcus sp. 'group G']]
+[[Category: Allain FH]]
+[[Category: Hargous YF]]