2fnb: Difference between revisions

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-[[Image:2fnb.jpg|left|200px]]
-<!--
+==NMR STRUCTURE OF THE FIBRONECTIN ED-B DOMAIN, NMR, 20 STRUCTURES==
-The line below this paragraph, containing "STRUCTURE_2fnb", creates the "Structure Box" on the page.
+<StructureSection load='2fnb' size='340' side='right'caption='[[2fnb]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2fnb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FNB FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fnb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fnb OCA], [https://pdbe.org/2fnb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fnb RCSB], [https://www.ebi.ac.uk/pdbsum/2fnb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fnb ProSAT]</span></td></tr>
-{{STRUCTURE_2fnb|  PDB=2fnb  |  SCENE=  }}
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/FINC_HUMAN FINC_HUMAN] Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2) [MIM:[https://omim.org/entry/601894 601894]; also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life.<ref>PMID:18268355</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/FINC_HUMAN FINC_HUMAN] Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.<ref>PMID:8114919</ref> <ref>PMID:11209058</ref> <ref>PMID:15665290</ref> <ref>PMID:19379667</ref>   Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.<ref>PMID:8114919</ref> <ref>PMID:11209058</ref> <ref>PMID:15665290</ref> <ref>PMID:19379667</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/2fnb_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fnb ConSurf].
+<div style="clear:both"></div>
-'''NMR STRUCTURE OF THE FIBRONECTIN ED-B DOMAIN, NMR, 20 STRUCTURES'''
+==See Also==
+*[[Fibronectin 3D structures|Fibronectin 3D structures]]
+== References ==
-==Overview==
+<references/>
-BACKGROUND: The process of angiogenesis (i.e. the formation of new blood vessels from pre-existing ones) is fundamental to physiological processes such as reproduction, development and repair, as well as to pathological conditions such as tumor progression, rheumathoid arthritis and ocular disorders. The oncofoetal ED-B domain, a specific marker of angiogenesis, consists of 91 amino acid residues that are inserted by alternative splicing into the fibronectin (FN) molecule. RESULTS: The NMR structure of the ED-B domain is reported and reveals important differences from other FN type III domains. A comparison of the ED-B domain with the crystal structure of a four-domain FN fragment shows the novel features of ED-B to be located in loop regions that are buried at interdomain interfaces, and which therefore largely determine the global shape of the FN molecule. The negatively charged amino acids in this highly acidic protein are uniformly distributed over the molecular surface, with the sole exception of a solvent-exposed hydrophobic patch that represents a potential specific recognition site. Epitope mapping with 82 decapeptides that span the ED-B sequence revealed that three ED-B-specific monoclonal antibodies, which selectively target newly forming blood vessels in tumor-bearing mice, bind to adjacent regions on the ED-B surface. CONCLUSIONS: The NMR structure enables the identification of a large surface area of the ED-B domain that appears to be accessible in vivo, opening up new diagnostic and therapeutic opportunities. Furthermore, the mapping of specific monoclonal antibodies to the three-dimensional structure of the ED-B domain, and their use in angiogenesis inhibition experiments, provides a basis for further investigation of the role of the ED-B domain in the formation of new blood vessels.
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Homo sapiens]]
-FNB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FNB OCA].
+[[Category: Large Structures]]
+[[Category: Fattorusso R]]
-==Reference==
+[[Category: Neri D]]
-NMR structure of the human oncofoetal fibronectin ED-B domain, a specific marker for angiogenesis., Fattorusso R, Pellecchia M, Viti F, Neri P, Neri D, Wuthrich K, Structure. 1999 Apr 15;7(4):381-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10196121 10196121]
+[[Category: Neri P]]
-[[Category: Escherichia coli]]
+[[Category: Pellecchia M]]
-[[Category: Single protein]]
+[[Category: Viti F]]
-[[Category: Fattorusso, R.]]
+[[Category: Wuthrich K]]
-[[Category: Neri, D.]]
-[[Category: Neri, P.]]
-[[Category: Pellecchia, M.]]
-[[Category: Viti, F.]]
-[[Category: Wuthrich, K.]]
-[[Category: Angiogenesis]]
-[[Category: Ed-b]]
-[[Category: Fibronectin]]
-[[Category: Typeiii domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:05:51 2008''