2fh2: Difference between revisions

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-[[Image:2fh2.gif|left|200px]]<br />
-<applet load="2fh2" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2fh2, resolution 2.500&Aring;" />
-'''C-terminal half of gelsolin soaked in EGTA at pH 4.5'''<br />
-==Overview==
+==C-terminal half of gelsolin soaked in EGTA at pH 4.5==
-Gelsolin is a calcium and pH-sensitive modulator of actin filament length., Here, we use X-ray crystallography to examine the extraction and exchange, of calcium ions from their binding sites in different crystalline forms of, the activated N and C-terminal halves of gelsolin, G1-G3 and G4-G6, respectively. We demonstrate that the combination of calcium and low pH, activating conditions do not induce conformational changes in G4-G6 beyond, those elicited by calcium alone. EGTA is able to remove calcium ions bound, to the type I and type II metal ion-binding sites in G4-G6. Constrained by, crystal contacts and stabilized by interdomain interaction surfaces, the, gross structure of calcium-depleted G4-G6 remains that of the activated, form. However, high-resolution details of changes in the ion-binding sites, may represent the initial steps toward restoration of the arrangement of, domains found in the calcium-free inactive form of gelsolin in solution., Furthermore, bathing crystals with the trivalent calcium ion mimic, Tb3+, results in anomalous scattering data that permit unequivocal localization, of terbium ions in each of the proposed type I and type II ion-binding, sites of both halves of gelsolin. In contrast to predictions based on, solution studies, we find that no calcium ion is immune to exchange.
+<StructureSection load='2fh2' size='340' side='right'caption='[[2fh2]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2fh2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FH2 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fh2 OCA], [https://pdbe.org/2fh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fh2 RCSB], [https://www.ebi.ac.uk/pdbsum/2fh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fh2 ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/GELS_HUMAN GELS_HUMAN] Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:[https://omim.org/entry/105120 105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.<ref>PMID:2157434</ref> <ref>PMID:2153578</ref> <ref>PMID:2176481</ref> <ref>PMID:1338910</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/GELS_HUMAN GELS_HUMAN] Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.<ref>PMID:20393563</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/2fh2_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fh2 ConSurf].
+<div style="clear:both"></div>
-==Disease==
+==See Also==
-Known disease associated with this structure: Amyloidosis, Finnish type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=137350 137350]]
+*[[Gelsolin 3D structures|Gelsolin 3D structures]]
+== References ==
-==About this Structure==
+<references/>
-FH2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FH2 OCA].
+__TOC__
+</StructureSection>
-==Reference==
-Calcium ion exchange in crystalline gelsolin., Chumnarnsilpa S, Loonchanta A, Xue B, Choe H, Urosev D, Wang H, Lindberg U, Burtnick LD, Robinson RC, J Mol Biol. 2006 Mar 31;357(3):773-82. Epub 2006 Jan 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16466744 16466744]
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Burtnick, L.D.]]
+[[Category: Burtnick LD]]
-[[Category: Choe, H.]]
+[[Category: Choe H]]
-[[Category: Chumnarnsilpa, S.]]
+[[Category: Chumnarnsilpa S]]
-[[Category: Loonchanta, A.]]
+[[Category: Loonchanta A]]
-[[Category: Robinson, R.C.]]
+[[Category: Robinson RC]]
-[[Category: Urosev, D.]]
+[[Category: Urosev D]]
-[[Category: Wang, H.]]
+[[Category: Wang H]]
-[[Category: Xue, B.]]
+[[Category: Xue B]]
-[[Category: CA]]
-[[Category: egta]]
-[[Category: gelsolin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:05:11 2007''