2f5h: Difference between revisions
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== | ==Solution structure of the alpha-domain of human Metallothionein-3== | ||
Alzheimer's disease is characterized by progressive loss of neurons | <StructureSection load='2f5h' size='340' side='right'caption='[[2f5h]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2f5h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F5H FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5h OCA], [https://pdbe.org/2f5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f5h RCSB], [https://www.ebi.ac.uk/pdbsum/2f5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f5h ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/MT3_HUMAN MT3_HUMAN] Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Alzheimer's disease is characterized by progressive loss of neurons accompanied by the formation of intraneural neurofibrillary tangles and extracellular amyloid plaques. Human neuronal growth inhibitory factor, classified as metallothionein-3 (MT-3), was found to be related to the neurotrophic activity promoting cortical neuron survival and dendrite outgrowth in the cell culture studies. We have determined the solution structure of the alpha-domain of human MT-3 (residues 32-68) by multinuclear and multidimensional NMR spectroscopy in combination with the molecular dynamic simulated annealing approach. The human MT-3 shows two metal-thiolate clusters, one in the N-terminus (beta-domain) and one in the C-terminus (alpha-domain). The overall fold of the alpha-domain is similar to that of mouse MT-3. However, human MT-3 has a longer loop in the acidic hexapeptide insertion than that of mouse MT-3. Surprisingly, the backbone dynamics of the protein revealed that the beta-domain exhibits similar internal motion to the alpha-domain, although the N-terminal residues are more flexible. Our results may provide useful information for understanding the structure-function relationship of human MT-3. | |||
Solution structure and dynamics of human metallothionein-3 (MT-3).,Wang H, Zhang Q, Cai B, Li H, Sze KH, Huang ZX, Wu HM, Sun H FEBS Lett. 2006 Feb 6;580(3):795-800. Epub 2006 Jan 9. PMID:16413543<ref>PMID:16413543</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2f5h" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Cai | [[Category: Cai B]] | ||
[[Category: Huang | [[Category: Huang ZX]] | ||
[[Category: Li | [[Category: Li HY]] | ||
[[Category: Sun | [[Category: Sun HZ]] | ||
[[Category: Sze | [[Category: Sze KH]] | ||
[[Category: Wang | [[Category: Wang H]] | ||
[[Category: Wu | [[Category: Wu HM]] | ||
[[Category: Zhang | [[Category: Zhang Q]] | ||
