2b2c: Difference between revisions

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-[[Image:2b2c.gif|left|200px]]
-<!--
+==Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase==
-The line below this paragraph, containing "STRUCTURE_2b2c", creates the "Structure Box" on the page.
+<StructureSection load='2b2c' size='340' side='right'caption='[[2b2c]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B2C FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [https://pdbe.org/2b2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [https://www.ebi.ac.uk/pdbsum/2b2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2c ProSAT]</span></td></tr>
-{{STRUCTURE_2b2c|  PDB=2b2c  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/G5EFG3_CAEEL G5EFG3_CAEEL]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/2b2c_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b2c ConSurf].
+<div style="clear:both"></div>
-'''Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase'''
+==See Also==
+*[[Spermidine synthase 3D structures|Spermidine synthase 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned from the model nematode Caenorhabditis elegans. Biochemical characterisation of the recombinantly expressed protein revealed a high degree of similarity to other eukaryotic SPDS with the exception of a low affinity towards the substrate decarboxylated S-adenosylmethionine (Km = 110 microM) and a less pronounced feedback inhibition by the second reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C. elegans protein that carries a nematode-specific insertion of 27 amino acids close to its N-terminus was crystallized, leading to the first X-ray structure of a dimeric eukaryotic SPDS.
-==About this Structure==
-B2C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA].
-==Reference==
-Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase., Dufe VT, Luersen K, Eschbach ML, Haider N, Karlberg T, Walter RD, Al-Karadaghi S, FEBS Lett. 2005 Nov 7;579(27):6037-43. Epub 2005 Oct 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16226262 16226262]
 [[Category: Caenorhabditis elegans]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Spermidine synthase]]
+[[Category: Al-Karadaghi S]]
-[[Category: Al-Karadaghi, S.]]
+[[Category: Dufe VT]]
-[[Category: Dufe, V T.]]
+[[Category: Eschbach ML]]
-[[Category: Eschbach, M L.]]
+[[Category: Haider N]]
-[[Category: Haider, N.]]
+[[Category: Karlberg T]]
-[[Category: Karlberg, T.]]
+[[Category: Luersen K]]
-[[Category: Luersen, K.]]
+[[Category: Walter RD]]
-[[Category: Walter, R D.]]
-[[Category: Beta-alpha]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:45:48 2008''