1zro: Difference between revisions

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-[[Image:1zro.gif|left|200px]]
-<!--
-The line below this paragraph, containing "STRUCTURE_1zro", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
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-{{STRUCTURE_1zro|  PDB=1zro  |  SCENE=  }}
-'''Crystal structure of EBA-175 Region II (RII) crystallized in the presence of (alpha)2,3-sialyllactose'''
+==Crystal structure of EBA-175 Region II (RII) crystallized in the presence of (alpha)2,3-sialyllactose==
+<StructureSection load='1zro' size='340' side='right'caption='[[1zro]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1zro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZRO FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zro OCA], [https://pdbe.org/1zro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zro RCSB], [https://www.ebi.ac.uk/pdbsum/1zro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zro ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q25735_PLAFA Q25735_PLAFA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zr/1zro_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zro ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Erythrocyte binding antigen 175 (EBA-175) is a P. falciparum protein that binds the major glycoprotein found on human erythrocytes, glycophorin A, during invasion. Here we present the crystal structure of the erythrocyte binding domain of EBA-175, RII, which has been established as a vaccine candidate. Binding sites for the heavily sialylated receptor glycophorin A are proposed based on a complex of RII with a glycan that contains the essential components required for binding. The dimeric organization of RII displays two prominent channels that contain four of the six observed glycan binding sites. Each monomer consists of two Duffy binding-like (DBL) domains (F1 and F2). F2 more prominently lines the channels and makes the majority of the glycan contacts, underscoring its role in cytoadherence and in antigenic variation in malaria. Our studies provide insight into the mechanism of erythrocyte invasion by the malaria parasite and aid in rational drug design and vaccines.
-==Overview==
+Structural basis for the EBA-175 erythrocyte invasion pathway of the malaria parasite Plasmodium falciparum.,Tolia NH, Enemark EJ, Sim BK, Joshua-Tor L Cell. 2005 Jul 29;122(2):183-93. PMID:16051144<ref>PMID:16051144</ref>
-Erythrocyte binding antigen 175 (EBA-175) is a P. falciparum protein that binds the major glycoprotein found on human erythrocytes, glycophorin A, during invasion. Here we present the crystal structure of the erythrocyte binding domain of EBA-175, RII, which has been established as a vaccine candidate. Binding sites for the heavily sialylated receptor glycophorin A are proposed based on a complex of RII with a glycan that contains the essential components required for binding. The dimeric organization of RII displays two prominent channels that contain four of the six observed glycan binding sites. Each monomer consists of two Duffy binding-like (DBL) domains (F1 and F2). F2 more prominently lines the channels and makes the majority of the glycan contacts, underscoring its role in cytoadherence and in antigenic variation in malaria. Our studies provide insight into the mechanism of erythrocyte invasion by the malaria parasite and aid in rational drug design and vaccines.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-ZRO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZRO OCA].
+</div>
+<div class="pdbe-citations 1zro" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-Structural basis for the EBA-175 erythrocyte invasion pathway of the malaria parasite Plasmodium falciparum., Tolia NH, Enemark EJ, Sim BK, Joshua-Tor L, Cell. 2005 Jul 29;122(2):183-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16051144 16051144]
+*[[Erythrocyte binding antigen|Erythrocyte binding antigen]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Plasmodium falciparum]]
-[[Category: Single protein]]
+[[Category: Enemark EJ]]
-[[Category: Enemark, E J.]]
+[[Category: Joshua-Tor L]]
-[[Category: Joshua-Tor, L.]]
+[[Category: Sim BK]]
-[[Category: Sim, B K.]]
+[[Category: Tolia NH]]
-[[Category: Tolia, N H.]]
-[[Category: Binding]]
-[[Category: Dbl]]
-[[Category: Disease]]
-[[Category: Eba-175]]
-[[Category: Erythrocyte]]
-[[Category: Glycan]]
-[[Category: Glycophorin]]
-[[Category: Host]]
-[[Category: Invasion]]
-[[Category: Malaria]]
-[[Category: Rii]]
-[[Category: Sialic acid]]
-[[Category: Sialyllactose]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:59:13 2008''