2bm6: Difference between revisions

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New page: left|200px<br /> <applet load="2bm6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bm6, resolution 2.2Å" /> '''THE STRUCTURE OF MFP...
 
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[[Image:2bm6.gif|left|200px]]<br />
<applet load="2bm6" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2bm6, resolution 2.2&Aring;" />
'''THE STRUCTURE OF MFPA (RV3361C, C2221 CRYSTAL FORM). THE PENTAPEPTIDE REPEAT PROTEIN FROM MYCOBACTERIUM TUBERCULOSIS FOLDS AS A RIGHT-HANDED QUADRILATERAL BETA-HELIX.'''<br />


==Overview==
==The Structure of MfpA (Rv3361c, C2221 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.==
Fluoroquinolones are gaining increasing importance in the treatment of, tuberculosis. The expression of MfpA, a member of the pentapeptide repeat, family of proteins from Mycobacterium tuberculosis, causes resistance to, ciprofloxacin and sparfloxacin. This protein binds to DNA gyrase and, inhibits its activity. Its three-dimensional structure reveals a fold, which we have named the right-handed quadrilateral beta helix, that, exhibits size, shape, and electrostatic similarity to B-form DNA. This, represents a form of DNA mimicry and explains both its inhibitory effect, on DNA gyrase and fluoroquinolone resistance resulting from the protein's, expression in vivo.
<StructureSection load='2bm6' size='340' side='right'caption='[[2bm6]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2bm6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BM6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BM6 FirstGlance]. <br>
2BM6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]] with CS as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BM6 OCA]].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bm6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bm6 OCA], [https://pdbe.org/2bm6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bm6 RCSB], [https://www.ebi.ac.uk/pdbsum/2bm6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bm6 ProSAT]</span></td></tr>
A fluoroquinolone resistance protein from Mycobacterium tuberculosis that mimics DNA., Hegde SS, Vetting MW, Roderick SL, Mitchenall LA, Maxwell A, Takiff HE, Blanchard JS, Science. 2005 Jun 3;308(5727):1480-3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15933203 15933203]
</table>
[[Category: Mycobacterium tuberculosis]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/O50390_MYCTO O50390_MYCTO]  
[[Category: Blanchard, J.S.]]
== Evolutionary Conservation ==
[[Category: Hegde, S.S.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Maxwell, A.]]
Check<jmol>
[[Category: Mitchenall, L.A.]]
  <jmolCheckbox>
[[Category: Roderick, S.L.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bm/2bm6_consurf.spt"</scriptWhenChecked>
[[Category: Takiff, H.E.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Vetting, M.W.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: CS]]
  </jmolCheckbox>
[[Category: dna gyrase]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bm6 ConSurf].
[[Category: dna mimicry]]
<div style="clear:both"></div>
[[Category: fluroquinolone resistance]]
__TOC__
[[Category: pentapeptide repeat protein]]
</StructureSection>
[[Category: right-handed quadrilateral beta-helix]]
[[Category: Large Structures]]
 
[[Category: Mycobacterium tuberculosis H37Rv]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:48:12 2007''
[[Category: Blanchard JS]]
[[Category: Hegde SS]]
[[Category: Maxwell A]]
[[Category: Mitchenall LA]]
[[Category: Roderick SL]]
[[Category: Takiff HE]]
[[Category: Vetting MW]]

Latest revision as of 09:38, 1 May 2024

The Structure of MfpA (Rv3361c, C2221 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.The Structure of MfpA (Rv3361c, C2221 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.

Structural highlights

2bm6 is a 1 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O50390_MYCTO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

2bm6, resolution 2.20Å

Drag the structure with the mouse to rotate

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OCA
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