1vqb: Difference between revisions

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-[[Image:1vqb.jpg|left|200px]]
-<!--
+==GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)==
-The line below this paragraph, containing "STRUCTURE_1vqb", creates the "Structure Box" on the page.
+<StructureSection load='1vqb' size='340' side='right'caption='[[1vqb]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1vqb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_f1 Enterobacteria phage f1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bgh 1bgh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VQB FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vqb OCA], [https://pdbe.org/1vqb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vqb RCSB], [https://www.ebi.ac.uk/pdbsum/1vqb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vqb ProSAT]</span></td></tr>
-{{STRUCTURE_1vqb|  PDB=1vqb  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/G5P_BPF1 G5P_BPF1] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vq/1vqb_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vqb ConSurf].
+<div style="clear:both"></div>
-'''GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)'''
+==See Also==
+*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47--&gt;methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 A resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein-single-stranded DNA complex.
-==About this Structure==
-VQB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_f1 Enterobacteria phage f1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bgh 1bgh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQB OCA].
-==Reference==
-Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein., Skinner MM, Zhang H, Leschnitzer DH, Guan Y, Bellamy H, Sweet RM, Gray CW, Konings RN, Wang AH, Terwilliger TC, Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2071-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8134350 8134350]
 [[Category: Enterobacteria phage f1]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Bellamy, H.]]
+[[Category: Bellamy H]]
-[[Category: Gray, C W.]]
+[[Category: Gray CW]]
-[[Category: Guan, Y.]]
+[[Category: Guan Y]]
-[[Category: Konings, R N.H.]]
+[[Category: Konings RNH]]
-[[Category: Leschnitzer, D H.]]
+[[Category: Leschnitzer DH]]
-[[Category: Skinner, M M.]]
+[[Category: Skinner MM]]
-[[Category: Sweet, R M.]]
+[[Category: Sweet RM]]
-[[Category: Terwilliger, T C.]]
+[[Category: Terwilliger TC]]
-[[Category: Wang, A H.J.]]
+[[Category: Wang AH-J]]
-[[Category: Zhang, H.]]
+[[Category: Zhang H]]
-[[Category: Dna-binding protein]]
-[[Category: Gene v]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:47:31 2008''