1xkt: Difference between revisions

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New page: left|200px<br /> <applet load="1xkt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xkt, resolution 2.60Å" /> '''Human fatty acid sy...
 
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[[Image:1xkt.gif|left|200px]]<br />
<applet load="1xkt" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xkt, resolution 2.60&Aring;" />
'''Human fatty acid synthase: Structure and substrate selectivity of the thioesterase domain'''<br />


==Overview==
==Human fatty acid synthase: Structure and substrate selectivity of the thioesterase domain==
Human fatty acid synthase is a large homodimeric multifunctional enzyme, that synthesizes palmitic acid. The unique carboxyl terminal thioesterase, domain of fatty acid synthase hydrolyzes the growing fatty acid chain and, plays a critical role in regulating the chain length of fatty acid, released. Also, the up-regulation of human fatty acid synthase in a, variety of cancer makes the thioesterase a candidate target for, therapeutic treatment. The 2.6-A resolution structure of human fatty acid, synthase thioesterase domain reported here is comprised of two dissimilar, subdomains, A and B. The smaller subdomain B is composed entirely of, alpha-helices arranged in an atypical fold, whereas the A subdomain is a, variation of the alpha/beta hydrolase fold. The structure revealed the, presence of a hydrophobic groove with a distal pocket at the interface of, the two subdomains, which constitutes the candidate substrate binding, site. The length and largely hydrophobic nature of the groove and pocket, are consistent with the high selectivity of the thioesterase for palmitoyl, acyl substrate. The structure also set the identity of the Asp residue of, the catalytic triad of Ser, His, and Asp located in subdomain A at the, proximal end of the groove.
<StructureSection load='1xkt' size='340' side='right'caption='[[1xkt]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1xkt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XKT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xkt OCA], [https://pdbe.org/1xkt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xkt RCSB], [https://www.ebi.ac.uk/pdbsum/1xkt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xkt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FAS_HUMAN FAS_HUMAN] Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xk/1xkt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xkt ConSurf].
<div style="clear:both"></div>


==Disease==
==See Also==
Known diseases associated with this structure: Autoimmune lymphoproliferative syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134637 134637]], Autoimmune lymphoproliferative syndrome, type IA OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134637 134637]], Squamous cell carcinoma, burn scar-related, somatic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134637 134637]]
*[[Fatty acid synthase 3D structures|Fatty acid synthase 3D structures]]
 
__TOC__
==About this Structure==
</StructureSection>
1XKT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XKT OCA].
 
==Reference==
Human fatty acid synthase: structure and substrate selectivity of the thioesterase domain., Chakravarty B, Gu Z, Chirala SS, Wakil SJ, Quiocho FA, Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15567-72. Epub 2004 Oct 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15507492 15507492]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Chakravarty, B.]]
[[Category: Chakravarty B]]
[[Category: Chirala, S.S.]]
[[Category: Chirala SS]]
[[Category: Gu, Z.]]
[[Category: Gu Z]]
[[Category: Quiocho, F.A.]]
[[Category: Quiocho FA]]
[[Category: Wakil, S.J.]]
[[Category: Wakil SJ]]
[[Category: drug target]]
[[Category: human fatty acid synthase]]
[[Category: specificity]]
[[Category: structure]]
[[Category: thioesterase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:07:08 2007''

Latest revision as of 11:51, 14 February 2024

Human fatty acid synthase: Structure and substrate selectivity of the thioesterase domainHuman fatty acid synthase: Structure and substrate selectivity of the thioesterase domain

Structural highlights

1xkt is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FAS_HUMAN Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1xkt, resolution 2.60Å

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