1u0c: Difference between revisions

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[[Image:1u0c.gif|left|200px]]


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==Y33C Mutant of Homing endonuclease I-CreI==
The line below this paragraph, containing "STRUCTURE_1u0c", creates the "Structure Box" on the page.
<StructureSection load='1u0c' size='340' side='right'caption='[[1u0c]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1u0c]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0C FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
{{STRUCTURE_1u0c|  PDB=1u0c |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0c OCA], [https://pdbe.org/1u0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0c RCSB], [https://www.ebi.ac.uk/pdbsum/1u0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0c ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0c_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0c ConSurf].
<div style="clear:both"></div>


'''Y33C Mutatant of Homing endonuclease I-CreI'''
==See Also==
 
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites. Due to their biological function and high level of target specificity, these enzymes are under intense investigation as tools for gene targeting. These studies require that naturally occurring enzymes be redesigned to recognize novel target sites. Here, we report studies in which the homodimeric LAGLIDADG homing endonuclease I-CreI is altered at individual side-chains corresponding to contact points to distinct base-pairs in its target site. The resulting enzyme constructs drive specific elimination of selected DNA targets in vivo and display shifted specificities of DNA binding and cleavage in vitro. Crystal structures of two of these constructs demonstrate that substitution of individual side-chain/DNA contact patterns can occur with almost no structural deformation or rearrangement of the surrounding complex, facilitating an isolated, modular redesign strategy for homing endonuclease activity and specificity.
 
==About this Structure==
1U0C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0C OCA].
 
==Reference==
Isolation and characterization of new homing endonuclease specificities at individual target site positions., Sussman D, Chadsey M, Fauce S, Engel A, Bruett A, Monnat R Jr, Stoddard BL, Seligman LM, J Mol Biol. 2004 Sep 3;342(1):31-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313605 15313605]
[[Category: Chlamydomonas reinhardtii]]
[[Category: Chlamydomonas reinhardtii]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bruett, A.]]
[[Category: Bruett A]]
[[Category: Chadsey, M.]]
[[Category: Chadsey M]]
[[Category: Engel, A.]]
[[Category: Engel A]]
[[Category: Fauce, S.]]
[[Category: Fauce S]]
[[Category: Monnat, R.]]
[[Category: Monnat R]]
[[Category: Seligman, L M.]]
[[Category: Seligman LM]]
[[Category: Stoddard, B L.]]
[[Category: Stoddard BL]]
[[Category: Sussman, D.]]
[[Category: Sussman D]]
[[Category: Dna endonuclease i-crei]]
[[Category: Protein/dna]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:35:26 2008''

Latest revision as of 11:43, 14 February 2024

Y33C Mutant of Homing endonuclease I-CreIY33C Mutant of Homing endonuclease I-CreI

Structural highlights

1u0c is a 4 chain structure with sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DNE1_CHLRE Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1u0c, resolution 2.50Å

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