8ytu: Difference between revisions
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==Mipa-PETase from Micromonospora pattaloongensis== | |||
<StructureSection load='8ytu' size='340' side='right'caption='[[8ytu]], [[Resolution|resolution]] 1.34Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8ytu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_pattaloongensis Micromonospora pattaloongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8YTU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8YTU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.34Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ytu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ytu OCA], [https://pdbe.org/8ytu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ytu RCSB], [https://www.ebi.ac.uk/pdbsum/8ytu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ytu ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A1H3QT72_9ACTN A0A1H3QT72_9ACTN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Enzymes capable of breaking down polymers have been identified from natural sources and developed for industrial use in plastic recycling. However, there are many potential starting points for enzyme optimization that remain unexplored. We generated a landscape of 170 lineages of 1894 polyethylene terephthalate depolymerase (PETase) candidates and performed profiling using sampling approaches with features associated with PET-degrading capabilities. We identified three promising yet unexplored PETase lineages and two potent PETases, Mipa-P and Kubu-P. An engineered variant of Kubu-P outperformed benchmarks in terms of PET depolymerization in harsh environments, such as those with high substrate load and ethylene glycol as the solvent. | |||
Landscape profiling of PET depolymerases using a natural sequence cluster framework.,Seo H, Hong H, Park J, Lee SH, Ki D, Ryu A, Sagong HY, Kim KJ Science. 2025 Jan 3;387(6729):eadp5637. doi: 10.1126/science.adp5637. Epub 2025 , Jan 3. PMID:39745946<ref>PMID:39745946</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Hong | <div class="pdbe-citations 8ytu" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Park | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Micromonospora pattaloongensis]] | |||
[[Category: Hong H]] | |||
[[Category: Kim K-J]] | |||
[[Category: Park J]] | |||
[[Category: Seo H]] | |||
Latest revision as of 09:15, 15 January 2025
Mipa-PETase from Micromonospora pattaloongensisMipa-PETase from Micromonospora pattaloongensis
Structural highlights
FunctionPublication Abstract from PubMedEnzymes capable of breaking down polymers have been identified from natural sources and developed for industrial use in plastic recycling. However, there are many potential starting points for enzyme optimization that remain unexplored. We generated a landscape of 170 lineages of 1894 polyethylene terephthalate depolymerase (PETase) candidates and performed profiling using sampling approaches with features associated with PET-degrading capabilities. We identified three promising yet unexplored PETase lineages and two potent PETases, Mipa-P and Kubu-P. An engineered variant of Kubu-P outperformed benchmarks in terms of PET depolymerization in harsh environments, such as those with high substrate load and ethylene glycol as the solvent. Landscape profiling of PET depolymerases using a natural sequence cluster framework.,Seo H, Hong H, Park J, Lee SH, Ki D, Ryu A, Sagong HY, Kim KJ Science. 2025 Jan 3;387(6729):eadp5637. doi: 10.1126/science.adp5637. Epub 2025 , Jan 3. PMID:39745946[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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