6t0u: Difference between revisions

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<StructureSection load='6t0u' size='340' side='right'caption='[[6t0u]], [[Resolution|resolution]] 3.12&Aring;' scene=''>
<StructureSection load='6t0u' size='340' side='right'caption='[[6t0u]], [[Resolution|resolution]] 3.12&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6t0u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/little_yellow-shouldered_bat/Guatemala/060/2010(H17N10)) Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))] and [https://en.wikipedia.org/wiki/Influenza_B_virus Influenza B virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T0U FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T0U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.12&#8491;</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.12&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t0u OCA], [https://pdbe.org/6t0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t0u RCSB], [https://www.ebi.ac.uk/pdbsum/6t0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t0u ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t0u OCA], [https://pdbe.org/6t0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t0u RCSB], [https://www.ebi.ac.uk/pdbsum/6t0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t0u ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H6QM91_9INFA H6QM91_9INFA]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). Here, we visualize by cryoelectron microscopy the conformational dynamics of the polymerase during the complete transcription cycle from pre-initiation to termination, focusing on the template trajectory. After exiting the active site cavity, the template 3' extremity rebinds into a specific site on the polymerase surface. Here, it remains sequestered during all subsequent transcription steps, forcing the template to loop out as it further translocates. At termination, the strained connection between the bound template 5' end and the active site results in polyadenylation by stuttering at uridine 17. Upon product dissociation, further conformational changes release the trapped template, allowing recycling back into the pre-initiation state. Influenza polymerase thus performs transcription while tightly binding to and protecting both template ends, allowing efficient production of multiple mRNAs from a single vRNP.
A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase.,Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S Cell. 2020 Apr 16. pii: S0092-8674(20)30389-5. doi: 10.1016/j.cell.2020.03.061. PMID:32304664<ref>PMID:32304664</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6t0u" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Influenza B virus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Cusack S]]
[[Category: Cusack S]]
[[Category: Kouba T]]
[[Category: Kouba T]]
[[Category: Wandzik JM]]
[[Category: Wandzik JM]]

Latest revision as of 08:21, 25 September 2024

Bat Influenza A polymerase product dissociation complex using 44-mer vRNA template with intact oligo(U) sequenceBat Influenza A polymerase product dissociation complex using 44-mer vRNA template with intact oligo(U) sequence

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.12Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

6t0u, resolution 3.12Å

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