9bdc: Difference between revisions
New page: '''Unreleased structure''' The entry 9bdc is ON HOLD Authors: Herbine, K.H., Nayak, A.R., Temiakov, D. Description: Cryo-EM Structure of the TEFM bound Human Mitochondrial Transcriptio... |
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The | ==Cryo-EM Structure of the TEFM bound Human Mitochondrial Transcription Elongation Complex in a Closed Fingers Domain Conformation== | ||
<StructureSection load='9bdc' size='340' side='right'caption='[[9bdc]], [[Resolution|resolution]] 2.54Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9bdc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9BDC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9BDC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.54Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9bdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9bdc OCA], [https://pdbe.org/9bdc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9bdc RCSB], [https://www.ebi.ac.uk/pdbsum/9bdc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9bdc ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RPOM_HUMAN RPOM_HUMAN] DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.<ref>PMID:21278163</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The mechanism by which RNAP selects cognate substrates and discriminates between deoxy and ribonucleotides is of fundamental importance to the fidelity of transcription. Here, we present cryo-EM structures of human mitochondrial transcription elongation complexes that reveal substrate ATP bound in Entry and Insertion Sites. In the Entry Site, the substrate binds along the O helix of the fingers domain of mtRNAP but does not interact with the templating DNA base. Interactions between RNAP and the triphosphate moiety of the NTP in the Entry Site ensure discrimination against nucleosides and their diphosphate and monophosphate derivatives but not against non-cognate rNTPs and dNTPs. Closing of the fingers domain over the catalytic site results in delivery of both the templating DNA base and the substrate into the Insertion Site and recruitment of the catalytic magnesium ions. The cryo-EM data also reveal a conformation adopted by mtRNAP to reject a non-cognate substrate from its active site. Our findings establish a structural basis for substrate binding and suggest a unified mechanism of NTP selection for single-subunit RNAPs. | |||
Structural basis for substrate binding and selection by human mitochondrial RNA polymerase.,Herbine K, Nayak AR, Temiakov D Nat Commun. 2024 Aug 20;15(1):7134. doi: 10.1038/s41467-024-50817-9. PMID:39164235<ref>PMID:39164235</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Herbine | <div class="pdbe-citations 9bdc" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Herbine KH]] | |||
[[Category: Nayak AR]] | |||
[[Category: Temiakov D]] | |||