1tzn: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1tzn.gif|left|200px]]<br />
-<applet load="1tzn" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1tzn, resolution 4.30&Aring;" />
-'''Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore bound to the VWA domain of CMG2, an anthrax toxin receptor'''<br />
-==Overview==
+==Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore bound to the VWA domain of CMG2, an anthrax toxin receptor==
-After binding to cellular receptors and proteolytic activation, the, protective antigen component of anthrax toxin forms a heptameric prepore., The prepore later undergoes pH-dependent conversion to a pore, mediating, translocation of the edema and lethal factors to the cytosol. We describe, structures of the prepore (3.6 A) and a prepore:receptor complex (4.3 A), that reveal the location of pore-forming loops and an unexpected, interaction of the receptor with the pore-forming domain. Lower pH is, required for prepore-to-pore conversion in the presence of the receptor, indicating that this interaction regulates pH-dependent pore formation. We, present an example of a receptor negatively regulating pH-dependent, membrane insertion.
+<StructureSection load='1tzn' size='340' side='right'caption='[[1tzn]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
+== Structural highlights ==
-==Disease==
+<table><tr><td colspan='2'>[[1tzn]] is a 28 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._A2012 Bacillus anthracis str. A2012] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TZN FirstGlance]. <br>
-Known diseases associated with this structure: Fibromatosis, juvenile hyaline OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=608041 608041]], Hyalinosis, infantile systemic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=608041 608041]]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-==About this Structure==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tzn OCA], [https://pdbe.org/1tzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tzn RCSB], [https://www.ebi.ac.uk/pdbsum/1tzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tzn ProSAT]</span></td></tr>
-TZN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TZN OCA].
+</table>
+== Function ==
-==Reference==
+[https://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.
-Structure of heptameric protective antigen bound to an anthrax toxin receptor: a role for receptor in pH-dependent pore formation., Lacy DB, Wigelsworth DJ, Melnyk RA, Harrison SC, Collier RJ, Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13147-51. Epub 2004 Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15326297 15326297]
+== Evolutionary Conservation ==
-[[Category: Bacillus anthracis]]
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tz/1tzn_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tzn ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Bacillus anthracis str. A2012]]
 [[Category: Homo sapiens]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Collier, R.J.]]
+[[Category: Collier RJ]]
-[[Category: Lacy, D.B.]]
+[[Category: Lacy DB]]
-[[Category: Melnyk, R.A.]]
+[[Category: Melnyk RA]]
-[[Category: Wigelsworth, D.J.]]
+[[Category: Wigelsworth DJ]]
-[[Category: CA]]
-[[Category: MG]]
-[[Category: heptamer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:29:56 2007''