8vy7: Difference between revisions
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<table><tr><td colspan='2'>[[8vy7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8VY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8VY7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[8vy7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8VY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8VY7 FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.68Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.68Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1AEI:4-methyl-N-[( | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1AEI:4-methyl-~{N}-[2-(1~{H}-1,2,3,4-tetrazol-5-yl)phenyl]-6-(trifluoromethyl)pyrimidin-2-amine'>A1AEI</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vy7 OCA], [https://pdbe.org/8vy7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vy7 RCSB], [https://www.ebi.ac.uk/pdbsum/8vy7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vy7 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vy7 OCA], [https://pdbe.org/8vy7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vy7 RCSB], [https://www.ebi.ac.uk/pdbsum/8vy7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vy7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | |||
Bitter taste sensing is mediated by type 2 taste receptors (TAS2Rs (also known as T2Rs)), which represent a distinct class of G-protein-coupled receptors(1). Among the 26 members of the TAS2Rs, TAS2R14 is highly expressed in extraoral tissues and mediates the responses to more than 100 structurally diverse tastants(2-6), although the molecular mechanisms for recognizing diverse chemicals and initiating cellular signalling are still poorly understood. Here we report two cryo-electron microscopy structures for TAS2R14 complexed with G(gust) (also known as gustducin) and G(i1). Both structures have an orthosteric binding pocket occupied by endogenous cholesterol as well as an intracellular allosteric site bound by the bitter tastant cmpd28.1, including a direct interaction with the alpha5 helix of G(gust) and G(i1). Computational and biochemical studies validate both ligand interactions. Our functional analysis identified cholesterol as an orthosteric agonist and the bitter tastant cmpd28.1 as a positive allosteric modulator with direct agonist activity at TAS2R14. Moreover, the orthosteric pocket is connected to the allosteric site via an elongated cavity, which has a hydrophobic core rich in aromatic residues. Our findings provide insights into the ligand recognition of bitter taste receptors and suggest activities of TAS2R14 beyond bitter taste perception via intracellular allosteric tastants. | |||
Bitter taste receptor activation by cholesterol and an intracellular tastant.,Kim Y, Gumpper RH, Liu Y, Kocak DD, Xiong Y, Cao C, Deng Z, Krumm BE, Jain MK, Zhang S, Jin J, Roth BL Nature. 2024 Apr;628(8008):664-671. doi: 10.1038/s41586-024-07253-y. Epub 2024 , Apr 10. PMID:38600377<ref>PMID:38600377</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 8vy7" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Latest revision as of 13:03, 17 October 2024
CryoEM structure of Gi-coupled TAS2R14 with cholesterol and an intracellular tastantCryoEM structure of Gi-coupled TAS2R14 with cholesterol and an intracellular tastant
Structural highlights
Publication Abstract from PubMedBitter taste sensing is mediated by type 2 taste receptors (TAS2Rs (also known as T2Rs)), which represent a distinct class of G-protein-coupled receptors(1). Among the 26 members of the TAS2Rs, TAS2R14 is highly expressed in extraoral tissues and mediates the responses to more than 100 structurally diverse tastants(2-6), although the molecular mechanisms for recognizing diverse chemicals and initiating cellular signalling are still poorly understood. Here we report two cryo-electron microscopy structures for TAS2R14 complexed with G(gust) (also known as gustducin) and G(i1). Both structures have an orthosteric binding pocket occupied by endogenous cholesterol as well as an intracellular allosteric site bound by the bitter tastant cmpd28.1, including a direct interaction with the alpha5 helix of G(gust) and G(i1). Computational and biochemical studies validate both ligand interactions. Our functional analysis identified cholesterol as an orthosteric agonist and the bitter tastant cmpd28.1 as a positive allosteric modulator with direct agonist activity at TAS2R14. Moreover, the orthosteric pocket is connected to the allosteric site via an elongated cavity, which has a hydrophobic core rich in aromatic residues. Our findings provide insights into the ligand recognition of bitter taste receptors and suggest activities of TAS2R14 beyond bitter taste perception via intracellular allosteric tastants. Bitter taste receptor activation by cholesterol and an intracellular tastant.,Kim Y, Gumpper RH, Liu Y, Kocak DD, Xiong Y, Cao C, Deng Z, Krumm BE, Jain MK, Zhang S, Jin J, Roth BL Nature. 2024 Apr;628(8008):664-671. doi: 10.1038/s41586-024-07253-y. Epub 2024 , Apr 10. PMID:38600377[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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