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| <!--
| | ==DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE== |
| The line below this paragraph, containing "STRUCTURE_1par", creates the "Structure Box" on the page.
| | <StructureSection load='1par' size='340' side='right'caption='[[1par]], [[Resolution|resolution]] 2.60Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1par]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PAR FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1par FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1par OCA], [https://pdbe.org/1par PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1par RCSB], [https://www.ebi.ac.uk/pdbsum/1par PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1par ProSAT]</span></td></tr> |
| {{STRUCTURE_1par| PDB=1par | SCENE= }}
| | </table> |
| | | == Function == |
| '''DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE'''
| | [https://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22] This protein acts as a transcriptional repressor of its own gene arc and of gene ant. |
| | | __TOC__ |
| | | </StructureSection> |
| ==Overview== | | [[Category: Large Structures]] |
| Transcription of the ant gene during lytic growth of bacteriophage P22 (ref. 1) is regulated by the cooperative binding of two Arc repressor dimers to a 21-base-pair operator site. Here we report the co-crystal structure of this Arc tetramer-operator complex at 2.6 A resolution. As expected from genetic and structural studies and from the co-crystal structure of the homologous Escherichia coli MetJ repressor, each Arc dimer uses an antiparallel beta-sheet to recognize bases in the major groove. However, the Arc and MetJ complexes differ in several important ways: the beta-sheet-DNA interactions of Arc are far less symmetrical; DNA binding by Arc is accompanied by important conformational changes in the beta-sheet; and Arc uses a different part of its protein surface for dimer-dimer interactions.
| | [[Category: Salmonella virus P22]] |
| | | [[Category: Pabo CO]] |
| ==About this Structure== | | [[Category: Raumann BE]] |
| 1PAR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA].
| | [[Category: Rould MA]] |
| | | [[Category: Sauer RT]] |
| ==Reference== | |
| DNA recognition by beta-sheets in the Arc repressor-operator crystal structure., Raumann BE, Rould MA, Pabo CO, Sauer RT, Nature. 1994 Feb 24;367(6465):754-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8107872 8107872]
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| [[Category: Enterobacteria phage p22]] | |
| [[Category: Single protein]]
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| [[Category: Pabo, C O.]] | |
| [[Category: Raumann, B E.]] | |
| [[Category: Rould, M A.]] | |
| [[Category: Sauer, R T.]] | |
| [[Category: Double helix]] | |
| [[Category: Protein-dna complex]] | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:53:24 2008''
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