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New page: left|200px<br /> <applet load="1pwm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pwm, resolution 0.92Å" /> '''Crystal structure o...
 
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[[Image:1pwm.gif|left|200px]]<br />
<applet load="1pwm" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1pwm, resolution 0.92&Aring;" />
'''Crystal structure of human Aldose Reductase complexed with NADP and Fidarestat'''<br />


==Overview==
==Crystal structure of human Aldose Reductase complexed with NADP and Fidarestat==
The X-ray structures of human aldose reductase holoenzyme in complex with, the inhibitors Fidarestat (SNK-860) and Minalrestat (WAY-509) were, determined at atomic resolutions of 0.92 A and 1.1 A, respectively. The, hydantoin and succinimide moieties of the inhibitors interacted with the, conserved anion-binding site located between the nicotinamide ring of the, coenzyme and active site residues Tyr48, His110, and Trp111. Minalrestat's, hydrophobic isoquinoline ring was bound in an adjacent pocket lined by, residues Trp20, Phe122, and Trp219, with the bromo-fluorobenzyl group, inside the "specificity" pocket. The interactions between Minalrestat's, bromo-fluorobenzyl group and the enzyme include the stacking against the, side-chain of Trp111 as well as hydrogen bonding distances with residues, Leu300 and Thr113. The carbamoyl group in Fidarestat formed a hydrogen, bond with the main-chain nitrogen atom of Leu300. The atomic resolution, refinement allowed the positioning of hydrogen atoms and accurate, determination of bond lengths of the inhibitors, coenzyme NADP+ and, active-site residue His110. The 1'-position nitrogen atom in the hydantoin, and succinimide moieties of Fidarestat and Minalrestat, respectively, form, a hydrogen bond with the Nepsilon2 atom of His 110. For Fidarestat, the, electron density indicated two possible positions for the H-atom in this, bond. Furthermore, both native and anomalous difference maps indicated the, replacement of a water molecule linked to His110 by a Cl-ion. These, observations suggest a mechanism in which Fidarestat is bound protonated, and becomes negatively charged by donating the proton to His110, which may, have important implications on drug design.
<StructureSection load='1pwm' size='340' side='right'caption='[[1pwm]], [[Resolution|resolution]] 0.92&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1pwm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PWM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.92&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FID:(2S,4S)-2-AMINOFORMYL-6-FLUORO-SPIRO[CHROMAN-4,4-IMIDAZOLIDINE]-2,5-DIONE'>FID</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pwm OCA], [https://pdbe.org/1pwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pwm RCSB], [https://www.ebi.ac.uk/pdbsum/1pwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pwm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ALDR_HUMAN ALDR_HUMAN] Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pw/1pwm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pwm ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1PWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL, NAP and FID as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aldehyde_reductase Aldehyde reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.21 1.1.1.21] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PWM OCA].
*[[Aldose reductase 3D structures|Aldose reductase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Ultrahigh resolution drug design. II. Atomic resolution structures of human aldose reductase holoenzyme complexed with Fidarestat and Minalrestat: implications for the binding of cyclic imide inhibitors., El-Kabbani O, Darmanin C, Schneider TR, Hazemann I, Ruiz F, Oka M, Joachimiak A, Schulze-Briese C, Tomizaki T, Mitschler A, Podjarny A, Proteins. 2004 Jun 1;55(4):805-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15146479 15146479]
[[Category: Aldehyde reductase]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Darmanin, C.]]
[[Category: Darmanin C]]
[[Category: El-Kabbani, O.]]
[[Category: El-Kabbani O]]
[[Category: Hazemann, I.]]
[[Category: Hazemann I]]
[[Category: Joachimiak, A.]]
[[Category: Joachimiak A]]
[[Category: Mitschler, A.]]
[[Category: Mitschler A]]
[[Category: Oka, M.]]
[[Category: Oka M]]
[[Category: Podjarny, A.]]
[[Category: Podjarny A]]
[[Category: Ruiz, F.]]
[[Category: Ruiz F]]
[[Category: Schneider, T.R.]]
[[Category: Schneider TR]]
[[Category: Schulze-Briese, C.]]
[[Category: Schulze-Briese C]]
[[Category: Tomizaki, T.]]
[[Category: Tomizaki T]]
[[Category: CL]]
[[Category: FID]]
[[Category: NAP]]
[[Category: aldose reductase]]
[[Category: atomic resolution]]
[[Category: inhibitor binding]]
[[Category: ternary complex]]
[[Category: x-ray crystallography]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:47:52 2007''

Latest revision as of 16:28, 13 March 2024

Crystal structure of human Aldose Reductase complexed with NADP and FidarestatCrystal structure of human Aldose Reductase complexed with NADP and Fidarestat

Structural highlights

1pwm is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 0.92Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ALDR_HUMAN Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1pwm, resolution 0.92Å

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